不同时间下低温胁迫对油棕幼苗生理生化变化的影响

Effect of Low Temperature Stress on Physiological Changes in Oil Palm( Elaesis guineensis Jacg. ) Seedling under Different Time

为了研究不同时间的低温胁迫对油棕幼苗生理生化变化的影响,以8个月苗龄的油棕幼苗为材料,根据海南的气温变化规律,设置4个不同的最低温度处理,分别为28℃(CK处理),18℃(T1处理)、12℃(T2处理)、6℃(T3处理),分别处理0、7、14、21 d时取样。结果发现,随着温度的下降和低温胁迫时间的延长,幼苗叶片含水量、SOD活性不断下降;可溶性蛋白、可溶性糖含量、H2O2、MDA含量不断升高;POD、CAT、APX活性先上升而后下降;T2、T3处理2周后的幼苗其心叶都变褐色,且生长明显减缓。低温会对油棕幼苗造成了明显地氧化伤害,但油棕幼苗可能通过提高可溶性蛋白、可溶性糖等物质的含量,抗氧化酶维持一定活性的适应机制,以抵抗低温造成的氧化伤害,维持其正常生长。

We used eight months of oil palm seedlings as materials to temperature stress on physiological and biochemical changes of oil study the effect of different time of low palm seedling , and according to the temperature variation in Hainan, set up four different minimum temperature treatments including CK （natural temperature treatment）, 18℃ （T1 treatment）, 12℃ （T2 treatment） and 6℃ （T3 treatment） after 0, 7, 14, 21 d sampling, respectively. With the decreasing of temperature and the increasing of time, the oil palm seedling leaf water content and SOD activity continued to decrease, the soluble protein, sugar content, H2O2 and MDA content continued to increase, and POD, CAT, APX activity first increased and then decreased. Under T2, T3 the seedling leaf became brown and oil palm seedling growth slowed down significantly after two-week treatment. Low temperature caused obviously oxidative damage, but oil palm seedlings used adaptation mechanisms by increasing the contents of soluble protein, sugar and antioxidant enzyme activity to resist oxidative damage caused by low temperature for normal growth.