高渗盐水对脑出血大鼠血脑屏障通透性的影响

Effect of hypertonic saline on permeability of blood-brain barrier in a rat model of intracerebral hemorrhage

目的 探讨高渗盐水对脑出血大鼠血脑屏障通透性的影响.方法 健康成年雄性SD大鼠60只,8周龄,体重260～ 300 g,采用随机数字表法分为4组（n=15）：假手术组（S组）、假手术＋高渗盐水组（S＋HS组）、脑出血组（ICH组）、脑出血＋高渗盐水组（ICH＋HS组）.采用脑内注入未抗凝的自体动脉血50μl建立大鼠脑出血模型.术毕苏醒后神经功能缺损评分1～3分为模型制备成功的标准.于模型制备成功后48 h时,处死大鼠,取脑组织,测定脑水含量,采用Western blot法测定occludin表达水平.处死前静脉注射伊文氏蓝（EB）,处死后取脑组织,测定EB含量.结果 与S组比较,ICH组和ICH＋HS脑含水量和EB含量升高,occudin表达下调（P＜0.05）,S＋HS组上述指标差异无统计学意义（P＞0.05）;与ICH组比较,ICH＋HS组脑含水量和EB含量降低,occudin表达上调（P＜0.05）.结论 高渗盐水可抑制脑出血大鼠血脑屏障通透性升高,减轻脑水肿.

Objective To investigate the effect of hypertonic saline （HS） on the permeability of blood-brain barrier in a rat model of intracerebral hemorrhage （ICH）.Methods Sixty healthy male Sprague-Dawley rats, aged 8 weeks, weighing 260-300 g, were randomly divided into 4 groups （n=15 each） using a random number table： sham operation group （group S）, sham operation＋HS group （group HS） ,ICH group, and ICH＋HS group.ICH was commonly induced in anesthetized rats by intraparenchymal injection of autologous blood 50 μ1.The equal volume of normal saline was given instead in group S.The neurologic deficits were scored on a five-point scale, and a score of 1-3 indicated successful establishment of the model.At 48 h after establishment of the model, the rats were sacrificed, and brains were removed for determination of brain water content, expression of occludin in brain tissues （by Western blot） , and Evans blue content.Results Compared with group S, the brain water content and Evans blue content were significantly increased, and the expression of occludin was down-regulated in ICH and ICH＋HS groups, and no significant change was found in the indices mentioned above in group S＋HS.Compared with group ICH,the brain water content and Evans blue content were significantly decreased, and the expression of occludin was up-regulated in group ICH ＋HS.Conclusion HS can inhibit increase in the permeability of bloodbrain barrier, and reduce the cerebral edema in a rat model of ICH.