18β-甘草次酸吡啶酰胺衍生物体外抗肿瘤活性研究

Study on Anti-tumor Activity of 18β-glycyrrhetinic Acid Derivatives Containing Pyridine Carboxamide in vitro

本试验研究18β-甘草次酸吡啶酰胺衍生物6c、10c、11c对HeLa、SMMC-7721、SGC-7901细胞增殖的影响。利用MTT法检测18β-甘草次酸吡啶酰胺衍生物6c、10c、11c对HeLa、SMMC-7721、SGC-7901细胞的抑制率;利用Hoechst-33258细胞凋亡染色试剂盒检测细胞凋亡情况;用Annexin V-FITC/PI双染法检测细胞凋亡率;蛋白质免疫印迹法检测相关蛋白表达水平;通过检测Caspases-3的活性进一步说明用药组诱导细胞凋亡的情况。结果表明,18β-甘草次酸吡啶酰胺衍生物6c、10c、11c对HeLa、SMMC-7721、SGC-7901细胞增殖均有抑制作用及促凋亡作用,且呈浓度依赖性,经t检验均有统计学意义(P<0.05)。18β-甘草次酸吡啶酰胺衍生物6c、10c、11c可诱导HeLa、SMMC-7721、SGC-7901细胞凋亡。

The assay was aimed to study the effect of 18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10 cand 11con the proliferation of HeLa,SMMC-7721 and SGC-7901 cells.Detected the cellular inhibition rate of 18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10 cand 11cto HeLa,SMMC-7721 and SGC-7901 cells by MTT method;Detected the conditions of apoptosis by Hoechst-33258 Staining Kit;Detected the apoptosis rate by Annexin V-FITC/PI method;The related protein expression levels were detected by Western blotting;To further clarify the situation of apoptosis induced in treatment group by detecting the activity of Caspases-3.18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10 cand 11c had the inhibition and promoting apoptosis effects on the proliferation of HeLa,SMMC-7721 and SGC-7901 cells,and had the concentration dependence.The results all showed statistical significance in t test（P〈0.05）.18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10 cand 11ccould induce the apoptosis of HeLa,SMMC-7721 and SGC-7901 cells.