反复无创性肢体缺血后适应对大鼠脑缺血的保护作用

Protective effect of repeated noninvasive limb ischemia postconditioning on cerebral ischemia in rats

目的探讨反复无创性远隔缺血后适应(RPC)对大鼠脑缺血15d内的保护作用及机制。方法 90只雄性SD大鼠随机分为3组,假手术组(sham组)、缺血再灌注组(I/R组)和RPC组,每组30只,每组1、3、6、10和15d共5个时间点,每个时间点6只。RPC组每天给予1次无创性RPC干预,各组大鼠神经功能(NSS)评分,TUNEL法测大脑海马CA1区神经元凋亡,免疫化学法测高敏C反应蛋白(hs-CRP)表达。结果与I/R组比较,RPC组1、3、6、10和15dNSS评分均降低[(11.00±1.41)分vs(15.50±1.38)分、(11.17±0.98)分vs(15.17±0.98)分、(8.00±1.26)分vs(14.83±1.94)分、(7.17±0.98)分vs(12.67±1.03)分和(7.50±1.76)分vs(11.67±1.63)分,P<0.05];与I/R组比较,RPC组各时间点hs-CRP表达明显降低(P<0.05)。结论反复无创性RPC有脑保护作用,其机制与抑制神经元凋亡及炎性反应有关。

Objective To study the protective effect of repeated remote noninvasive limb ischemia postconditioning on cerebral ischemia in rats.Methods Ninety male SD rats were randomly divided into sham operation group,I/R injury group,and repeated postconditioning（RPC）group（30in each group）.Rats in RPC group received noninvasive limb ischemia postconditioning once a day.Nerve function in each group was scored.Apoptosis of neurons and hs-CRP level in hippocampal CA1 were measured.Results The nerve function score and serum hs-CRP level were significantly lower in RPC group than in I/R injury group on days 1,3,6,10,and 15（11.00±1.41 vs 15.50±1.38,11.17±0.98 vs 15.17±0.98,8.00±1.26 vs 14.83±1.94,7.17±0.98 vs 12.67±1.03,7.50±1.76 vs 11.67±1.63,P〈0.05）.Conclusion Repeated noninvasive limb ischemia postconditioning protects brain of rats by inhibiting apoptosis of neurons and inflammatory reaction.