阿维拉霉素发酵条件的研究

Fermentation conditions of avilamycin

利用绿色产色链霉菌进行液体发酵,比较培养基初始pH值、温度、溶氧条件、碳源和氮源等生长条件对阿维拉霉素发酵的影响。实验发现,当培养基初始pH值为7.0,培养温度为28.0℃,摇床转速为200r/min,葡萄糖为碳源,黄豆饼粉为氮源等条件下阿维拉霉素的发酵水平较高,最高达到2 536μg/ml,为工业化生产提供了可靠依据。

Streptomyces hygroscopicus was used for liquid fermentation . Five indicators were selected to compare their re- spective effects on the avilamycin fermentation including initial pH of medium, fermentation temperature, dissolved oxygen, car- bon source and nitrogen source. It was found that initial pH7.0 for the medium, fermentation temperature of 28.0℃, table speed of 200 r/min,glucose as carbon source and soybean cake powder as nitrogen source were the optimum processing condition of the avilamycin fermentation. In this condition,the content of avilamycin was able to reach 2 536 μg/ml and provided a reliable basis for industrialized production.