靶向CD44的shRNA片段逆转人胰腺癌细胞株PANC-1侵袭表型机制

Targeting CD44 suppresses cell proliferation and metastasis in human pancreatic cancer cells

目的:利用RNA干扰技术沉默人胰腺癌细胞株PANC-1中CD44的表达,观察其对肿瘤侵袭性的抑制效果。方法:设计合成有效地干扰CD44的shRNA序列。结果:通过平板克隆形成实验、软琼脂集落形成试验、Transwell小室侵袭实验发现,胰腺癌细胞株转染干扰CD44的shRNA序列后,其相关的增殖以及侵袭能力明显受抑制。Western Blot发现随着CD44的下调,AKT被逐渐上调,而p-ERK、p-AKT却被逐渐下调。结论:靶向CD44的shRNA技术可有效降低人胰腺癌细胞株PANC-1的侵袭能力,可能的机制与影响其下游的p-AKT、p-ERK表达有关。针对CD44的RNA干扰具有潜在的临床价值。

Objective：We investigated the potential functions of shRNA in CD44 targeting in pancreatic cancer cells. Methods：To devise and synthesis of effectively interference of shRNA sequence of CD44,which was transefect- ed to the pancreatic cancer cells. Results：After the transfection, pancreatic cancer cells proliferation and invasion a- bilities were obviously inhibited by the colony formation assay, soft agar colony formation test, Transwell chamber inva- sion assay. Western Blot showed that p - ERK, p - AKT was reduced and the AKT were gradually raised in protein levels. Conclusion：shRNA targets CD44 in pancreatic cancer cells and suppresses pancreatic cancer cell growth and metastasis in vitro. This project may provide highly effective targeted therapy for pancreatic cancer and provide a new idea and method for targeting, CD44.