期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

BP1基因对肾透明细胞癌细胞株RLC-310增殖、克隆形成及迁移能力的影响

Effects of recombinant plasmid of p EGFP-N1-BP1 on proliferation,clone formation and migration of RLC-310 cells
下载PDF
导出
摘要 目的:研究BP1基因对肾透明细胞癌细胞株RLC-310细胞增殖、克隆形成能力及迁移能力的影响。方法:将前期构建的质粒p EGFP-N1-BP1转染入RLC-310细胞系,用Real-time PCR和Western blot检测BP1 mRNA与蛋白的表达情况;通过MTT、平板克隆实验及Transwell实验检测p EGFP-N1-BP1对RLC-310细胞的作用。结果:转染了p EGFP-N1-BP1的RLC-310细胞中BP1基因/蛋白的表达明显上调;转染组较空白对照组细胞增殖能力明显增加(P<0.05),克隆能力及迁移能力增强(P<0.05)。结论:BP1基因能够增强RLC-310细胞的增殖、克隆形成能力及迁移能力。 Objective ：To explore the effects of pEGFP- N1 -BP1 on proliferation, clone formation and migration of RLC -310 cells. Methods：The pEGFP - N1 - BP1 was transfected into RLC - 310 cells. The expression of BP1 gene was detected by Real - time PCR and Western blot. The effects of proliferation, clone formation and migration of RLC -310 cells were examined by MTT, clone formation assay and Transwell assay respectively. Results：The RLC -310 cells transfected with pEGFP - N1 - BP1 plasmid expressed a higher level of Twist protein steadily. The capabili- ties of proliferation, clone formation and migration of RLC -310 cells increased significantly compared with blank con- trol group（P 〈 0.05 ）. Conclusion： BP1 gene can enhance the abilities of proliferation, clone formation and migration of RLC - 310 cells.
作者 帖鹏 杜双宽 任伟 段万里
机构地区 陕西省人民医院
出处 《现代肿瘤医学》 CAS 2015年第24期3564-3568,共5页 Journal of Modern Oncology
关键词 肾透明细胞癌 BP1 增殖 克隆形成 迁移 RCCC, BP1, proliferation, clone formation, migration
分类号 R737.12 [医药卫生—肿瘤]
  • 相关文献

参考文献22

  • 1李恩惠,夏术阶.晚期肾癌靶向治疗的现状与进展[J].现代肿瘤医学,2012,20(7):1514-1518. 被引量：19
  • 2Razmkhah M, Jaberipour M, Ghaderi A. Bcl - 2 and Fas expres- sions correlate with proliferative specificity of adipose - derivcd stem cells(ASCs) in breast cancer [ J ]. lmmunol Invest, 2011,40 (3) :290 - 298.
  • 3Adeniran AJ,Zhu Z, Gandhi M, et al. Correlation betwcen gcnclic aherations and microscopic features, clinical manifestations, and prognostic characteristics of thyroid papillary carcinomas[ J ]. AM J Surg Pathol, 2006,30 ( 2 ) :216 - 222.
  • 4Knauf JA, Sartor MA, Medvedovic M, et al. Progression of BRAF - induced thyroid cancer is associated with epithelial - mesenchymal transition requiring concomitant MAP kinase and TGFβ signaling [J]. Oncogene,2011,30(28) :3153 -3162.
  • 5贺赛,郑见宝,孙学军,陈南征,周培华,魏光兵,王晖,姚建锋,张立,贾蓬勃.5HRE增强子和hTERT启动子联合调控CDX2基因对人结肠癌细胞系LoVo增殖的抑制[J].中国肿瘤生物治疗杂志,2014,21(3):314-319. 被引量：4
  • 6Santarpia L, Myers JN, Sherman SI, et al. Genetic alterations in the RAS/RAF/mitogen - activated protein kinase and phosphatidyli- nositol 3 - kinanse/Akt signaling pathways in the follicular variant of papillary thyroid carcinoma[ J]. Cancer,2010,116 (12) :2974 - 2983.
  • 7S Durand C, Ferraro - Pey RET, Selmi - Ruby, et al. Evaluation of gene expression profiles in thyroid nodule biopsy material to diag- nose thyroid cancer [ J ]. J Clin Endocrinol Metab, 2008,93 ( 4 ) : 1195 - 1202.
  • 8Yasuoka H, Nakamura Y, Zuo H, et al. VEGF - D expression and lymph vessels play an important role for lymphnode metastasis in papillary thyroid carcinoma [ J ]. Mod Pathol,2005,18 ( 8 ) : 1127 - 1133.
  • 9Man YG, Schwart ZA, Levine PH, et al. BP1, a putative sig - nature marker for inflammatory breast cancer and tumor aggres - siveness [ J]. Cancer Biomark,2009,5 ( 1 ) :9 - 17.
  • 10Cavalli LR, Man JG, Schwartz AM, et al. Amplification of the BP1 homebox gene in breast cancer [ J ]. Cancer Genet Cytogenet, 2008,187(1) :19 -24.

二级参考文献27

  • 1Krause,DS,Van,Etten,RA,黄艳.癌症治疗的靶点——酪氨酸激酶[J].中国处方药,2005(10):33-36. 被引量：45
  • 2Laszlo L. Predictive and prognostric factors in the complex treatment of patients with colorectal cancer [J]. Magy Onkol, 2010, 54 (4) : 383-394.
  • 3Shayakhmetov DM, DI Paolo NC, Mossman KL. Recognition of virus infection and innate host responses to viral gene therapy vee- tors [J]. Mol Ther, 2012,18(8): 1422-1429.
  • 4Chen EQ, Song XQ, Wang YL, et al. Construction of a highlyactive, liver-specific transcriptional regulatory element through combination of the albumin promoter and a-fetoprotein enhancer [J]. Plasmid, 2011,65(2): 125-131.
  • 5Dong K, Wang R, Wang X, et al. Tumor-specific RNAi targeting eIF4E suppresses tumor growth, induces apoptosis and enhances cisplatin cytotoxicity in human breast carcinoma cells [J]. Breast Cancer Res Treat, 2009,113(3): 443456.
  • 6Asayama M, Kadowaki S, Yamaguchi K. Issues of molecular targeted therapies in combination with chemotherapy in metastatic colorectal cancer [J]. Nihon Rinsho, 2011 , 69 ( 3) : 464 - 471.
  • 7Liu J, Harada H, Ogura M, et al. Adenovirus-mediated hypoxiatargeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts [J]. BrJ Cancer, 2007, 96( 12) : 1871-1878.
  • 8Zeng H, Wei Q, Huang R, et al. Recombinant adenovirus mediated prostate-specific enzyme pro-drug gene therapy regulated by prostate-specific membrane antigen ( PSMA) enhancer/promoter [J]. J Androl , 2007, 28 (6) : 827-835.
  • 9Chen L, Tang XD, Yu ST, et al. Induction of anti -tumor immunity by dendritic cells transduced with hTERT recombinant adenovirus in mice [J]. J Pathol, 2009, 217(5): 685-692.
  • 10Xu Y, Hou J, Liu Z, et al. Gene therapy with tumor-specific promoter mediated suicide gene plus IL-12 gene enhanced tumor inhibition and prolonged host survival in a murine model of Lewis lung carcinoma [J]. J Transl Med, 2011 , 9 ( 1 ) : 39.

共引文献21

现代肿瘤医学
2015年 第24期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部