用Chiralpak AD-H手性色谱柱HPLC法拆分阿罗洛尔对映体

Enantiomeric separation of arotinolol by Chiralpak AD-H chiral column HPLC method

目的:建立拆分阿罗洛尔手性对映体的手性固定相HPLC法。方法:采用纤维素直链淀粉类手性固定相Chiralpak AD-H色谱柱(250mm×4.6mm,5μm),考察了流动相比例、流速、碱性添加剂等因素对阿罗洛尔手性对映体拆分效果的影响。结果:确定了最佳拆分条件:流动相为正己烷-乙醇-二乙胺-三乙胺=30∶70∶0.1∶0.1(V/V),流速为0.4ml/min,检测波长为315nm,柱温为25℃,阿罗洛尔对映体拆分的分离度为3.033,对映体能够得到良好的拆分。结论:该法操作简便,可用于阿罗洛尔对映体的分离及质量控制。

Objective： To establish a chiral stationary phase HPLC method for the enantiomeric separation of arotinolol. Methods： An amylose chiral stationary phase Chiralpak AD-H column （250 min× 4.6 mm,5 μm） was used. Effects of the pro- portion of the mobile phase, flow rate and alkaline additives on the separation of arotinolol enantiomers were investigated. Results：The optimized chromatographic conditions were as follows： the mobile phase was hexane ： ethanol ： diethylamine ： triethylamine：30 ： 70 ： 0. ] ： 0. I（V/V） with a flow rate of 0.4 ml/min and the detection wavelength of 315 nm. The column temperature was set at 25 ~C. The arotinolol enantiomers could be well separated and the resolution was 3. 033. Conclusion： This method is simple to handle and it could be used for the separation and quality control of arotinolol enantiomers.