用双波长紫外分光光度法测定尿囊素维E乳膏中维生素E的含量

Determination of vitamin E in allantion vitamin E cream by dual wavelength UV-spectrophotometric method

目的：建立测定尿囊素维E乳膏中维生素E含量的双波长紫外分光光度法。方法：采用紫外一可见分光光度计测定维生素E的含量，测定波长为285nm，参比波长为293．2nm。结果：维生素E在31．96～95．88μg／ml范围内线性关系良好（r-O．9999），平均回收率为100．80％，RSD为1．21％（n=-9）。结论：该方法准确、灵敏，专属性强，重现性好，对尿囊素维E乳膏质量控制标准的提高具有参考意义。

Objective： To establish a dual wavelength UV-spectrophotometric method for the determination of vitamin E in allantion vitamin E cream. Methods： The UV-visible spectrophotometer was used for content determination of vitamin E. The detection wavelength and reference wavelength were 285 nm and 293.2 nm,respectively. Results：The linear range of vitamin E was 31.96-95.88 μg/ml（r=0. 999 9）. The average recovery was 100.80G ,RSD was 1.21% （n= 9）. Conclusion： The method was more accurate, sensitive, specific and reproducible. It could provide the reference for the improvement of the quality stand- ards of allantion vitamin E cream.