控制性皮质撞击法建立大鼠脑外伤模型的昏迷及认知障碍观察

Modeling Traumatic Brain Injury Rats with Controlled Cortical Impact: Coma and Cognitive Dysfunction

目的探讨控制性皮质撞击(CCI)法建立大鼠脑外伤模型的昏迷、神经行为学和认知功能表现。方法 40只雄性Sprague-Dawley大鼠随机分成对照组(n=10)、假手术组(n=10)、CCI组(n=20)。CCI组大鼠进行双侧额叶打击,打击速度3.5 m/s,深度7 mm,停留时间250 ms。记录昏迷时间。于术后10 d、20 d分别进行水迷宫测试。结果 CCI组术后意识障碍时间较对照组和假手术组延长(P<0.05)。术后10 d、20 d,CCI组逃避潜伏期均较对照组和假手术组显著延长(P<0.001),目标象限停留时间百分比显著减少(P<0.001)。结论大鼠双侧额叶CCI可以建立重度脑外伤昏迷动物模型,对认知障碍产生长期影响。

Objective To establish the model of traumatic brain injury in rats with controlled cortical impact（CCI）, and investigate the features of coma and cognition. Methods 40 male Sprague-Dawley rats were randomly divided into control group（n=10）, sham group（n=10） and CCI group（n=20）. CCI group was impacted at bilateral frontal lobe with the velocity of 3.5 m/s, depth of 7 mm and dwell time of250 ms. The time of consciousness loss was recorded, and all the rats were investigated with Morris Water Maze 10 and 20 days after operation. Results The time of consciousness loss was longer in the CCI group than in the control group and the sham group（P〈0.001）. The escape latency was longer in the CCI group than in the control group and the sham group（P〈0.001）, while the percent of time spend in goal quarter during probe trial was less（P〈0.001）, both 10 days and 20 days after operation. Conclusion CCI at bilateral frontal lobe of rats can establish the model of severe traumatic brain injury with coma and long-term cognitive dysfunction.