大川芎方HPLC指纹图谱方法学研究

The HPLC Fingerprint Analysis of Dachuanxiong Formula

建立大川芎方HPLC指纹图谱方法学。考察色谱条件,选择合适的分析波长,考察方法的精密度、重现性、稳定性等。结果表明:色谱条件:色谱柱:Hypersil ODS2 C18(4.6 mm×250 mm,5μm)反相色谱柱,流动相甲醇-1%醋酸梯度洗脱:0 min→35 min,A:5%→35%;35 min→50 min,A:35%→80%;50 min→60 min,A:80%→80%;60 min→65 min,A:80%→5%;柱温:30℃;流速:1 m L/min;检测波长270 nm。在上述条件下,所建立方法的精密度、重现性、稳定性等良好。所建立的大川芎方HPLC指纹图谱方法学为该方的定性、定量分析提供了新方法。

To developHPLC chromatographic fingerprint for Dachuanxiong formula. The chromatographic conditions were investigated, the proper analytical wavelength was inspected, and the precision,reproducibility and stability were determined. Chromatographic conditions： A Hypersil ODS2 C18（4.6 mm ×250 mm,5 μm）was applied with the gradient elution solvent system composing of methanol（A） and 1 % acetic acid water（B）： 0 min→35 min,A：5 %→35 %;35 min→50 min,A：35 %→80 %;50 min→60 min,A：80 %→80 %;60 min→65 min,A：80 %→5 %. The column temperature was 30 ℃ and the flow rate was 1 mL/min.The signal was acquired at 270 nm. Under the conditions we investigated, the precision, reproducibility and stability of the HPLC chromatographic fingerprint method were good. The HPLC chromatographic fingerprint method established in this paper applied a new method for qualitative and quantitative for Dachuanxiong formula.