摘要
采用转基因大豆GTS40-3-2品系特异性基因LAMP引物,建立2种转基因大豆GTS40-3-2品系特异性基因等温扩增方法:(1)实时荧光环介导等温扩增方法;(2)反应前加入染料羟基萘酚蓝(HNB)作为LAMP扩增的指示剂,根据反应后HNB的颜色变化进行判定。对两种方法进行灵敏度、特异性测试,结果表明,两种方法皆能快速、特异性检测出转基因大豆GTS40-3-2品系成分,定性检测限为0.1%。基于颜色判定的LAMP检测方法对设备要求更简单,更适用于日常检验及监管部门进行转基因成分的快速检测。
According to the specific loop-mediated isothermal amplification （LAMP） primers, two specific LAMP methods for the detection of the transgenic soybean GTS40-3-2 strain were established： （1） real-time fluorescence loop-mediated isothermal amplification （RT-LAMP）; （2） LAMP with hydroxy naphthol blue （HNB） dye as indicator. The specificity and sensitivity of these two methods were evaluated. The results indicated that the transgenic soybean GTS40-3-2 could be distinguished from several GM events and non-GM plants with limitation of dection of 0.1%. The LAMP assay with HNB dye requires only simple laboratory equipment and it is suitable for daily inspection and supervision departments for rapid detection of genetically modified ingredients.
出处
《亚热带植物科学》
2015年第3期213-217,共5页
Subtropical Plant Science
基金
湖南省质量技术监督局科研项目(2013KJJH11)
