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哈瓦那辣椒悬浮培养的体细胞胚发生

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摘要 本文对不同培养基、不同外植体类型(节点、节间、下胚轴、半粒种子、果段)诱导哈瓦那辣椒产生体细胞胚胎的情况进行了评估。在添加9.05μM 2,4-D、3%蔗糖和0.8%植物凝胶的MS基本培养基中,25±2℃,连续30d光照(40~50μmol·m^2/s)条件下,诱导胚性愈伤组织。形成的愈伤组织将被转移到组成相同的液体培养基中。仅当以下胚轴外植体,用含3.4IxM噻苯隆的MS培养基对其进行诱导时,才能产生体细胞胚。本研究建立了辣椒“细胞悬浮培养的体细胞胚发生”的一种实验方案。
出处 《辣椒杂志》 2015年第3期44-50,共7页 Journal of China Capsicum
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  • 1Agrawal S, Chandra N. 1983. Differentiation of multiple shoot buds and plantlets in cultured embryos of Capsicum annuum L. var. Mathania. Cun. Sci. 52:645 - 646.
  • 2Arroyo R,Revilla M A. 1991. In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars. Plant Cell Rep. 10:414 - 416.
  • 3Binzel M L, Sankhla N, Joshi S, Sankhla D. 1996. Induction of direct somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.). Plant Cell Rep. 15:536 - 540.
  • 4Binzel M L, Sankhla N, Joshi S, Sankhla D. 1996. In vitro regeneration in chile pepper ( Capsicum annuum L.) from ‘ half-seed explants. ’ Plant Growth Regulat. 20:287 - 293.
  • 5Buyukalaca S, Mavituna F. 1996. Somatic embryogenesis and plant regeneration of pepper in liquid media. Plant Cell Tiss. Org. Cult. 46:227 - 235.
  • 6Ezura H, Nishimiya S, Kasumi M. 1993. Efficient regeneration of plants independent of exogenous growth regulators in bell pepper ( Capsicum annuum L.). Plant Cell Rep. 12:676 - 680.
  • 7Fari M, Czako M. 1981. Relationships between position and morphogenetic response of pepper hypocotyls explant cultured in vitro. Sci. Hort. 15:207 - 213.
  • 8Ge K L, Sasakuma T, Tanaka M. 1998. Callus induction and plant regeneration in Capsicum. Acta Bot. Sin. 31:962 -965.
  • 9Gunay A L, Rao P S. 1978. In vitro plant regeneration from hypocotyls and cotyledon explants of red pepper (Capsicum). Plant Sci.Lett. 11:365 - 372.
  • 10Phillips G C,Hubstenberger J F. 1985. Organogenesis in pepper tissue cultures. Plant Cell Tiss. Org. Cult. 4:261 -269.

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