HPLC法同时测定保健食品中维生素A和维生素E

Simultaneous determination of vitamin A and vitamin E in health foods by HPLC

建立了高效液相色谱法（HPLC）同时测定保健食品中VA、VE的方法。样品预处理采用经二甲亚砜和正已烷的混合溶液于60℃剧烈振摇提取30rain，离心后吸取正己烷层，合并3次提取的溶液后直接进行液相色谱测定。方法采用Inertsil氨基柱（4．6mm×250mm，5μm），以含0．1％异丙醇的正己烷为流动相进行等度洗脱，流速为1．0mL／min，检测波长为280nm，柱温为25℃。结果表明，VA、VE分别在5～25μg／mL、100～500μg／mL范围内线性关系良好，相关系数分别达0．9997和0．9998，保留时间和峰面积的日内和日间精密度均较好，平均加标回收率在97．53％～100．67％之间，相对标准偏差（RSD）均小于2．0％。与相应的检验标准测定结果比较，相对偏差均小于1．0％，无明显差异。该方法快速、准确、可靠，适用于不同厂家、不同配方的保健食品中添加合成VA、VE的同时定量测定。

A method based on high performance liquid chromatography （HPLC） for simultaneous determination of vitamin A and vitamin E in health foods was established. These two kinds of vitamin above were extracted in the mixture of dimethyl sulfoxide and hexane by shaking at 60℃for 30 minutes. The mixture was centrifuged and the portion of hexane was obtained conveniently. Inertsil NH2 column （4.6 mm × 250 mm, 5 μm） was used with mobile phase of hexane （0.1% isopropanol） and isocratic elution was conducted. The detection wavelength was 280nm, and the column temperature was 25 ℃ . The method was proved to be linear when concentrations of Vitamin A and vitamin E were in range of 5 - 25 μg/mL and 100 - 500 μg/mL with the correlation coefficients 0.9997 and 0.9998 respectively. The intra and inter day precision of retention time and peak area were also good. The recovery rate was 97.53% - 100.67%, and RSD was less than 2.0%. The relative deviation of the results between the method established in this paper and the standard was less than 1.0%. The method is convenient, accurate, reliable and suitable for the determination of vitamin A and vitamin E in health foods at the same time. The simultaneous determination of synthetic vitamin A and vitamin E of different manufacturers and formulations was achieved.