胰腺癌组织中PTEN蛋白表达及细胞周期分析

THE EXPRESSION OF PTEN PROTEIN AND ANALYSIS OF CELL-CYCLE IN PANCREATIC CARCINOMA TISSUES

目的研究胰腺癌组织中PTEN蛋白表达以及细胞周期的变化,探讨其与肿瘤发生发展的关系。方法采用流式细胞术检测32例正常胰腺黏膜、76例胰腺癌组织中PTEN蛋白表达和细胞周期变化。结果从正常胰腺黏膜到胰腺癌组织中PTEN蛋白表达逐渐降低,统计学分析表明PTEN蛋白在胰腺癌组织中的表达与正常组比较差异有统计学意义(χ2=41.58,P<0.01)。胰腺癌组织的S期组分高于对照组,与对照组比较差异有统计学意义(t=9.63,P<0.01)。结论 PTEN蛋白表达异常与细胞周期调控障碍可能在胰腺癌发病过程中发挥重要的作用。

Objective To study the expression of PTEN protein and the changes of cell-cycle,and its relationship with the tumorigensis of pancreatic carcinoma.Methods Totally 32 cases of normal pancreatic mucosa and 76 cases of pancreatic carcinoma tissues were examined for PTEN protein expression and the change of cell-cycle by flow cytometry.Results From normal pancreatic mucosa tissues to pancreatic carcinoma,the expression of PTEN protein gradually decreased.The expression of PTEN protein in the pancreatic carcinoma group compared with normal control group,there was statistically signficant difference（χ2=41.58,P 〈0.01）.The analysis of cell-cycle showed that S phase fraction gradually increased from normal pancreatic mucosa to pancreatic carcinoma.The S phase fraction in pancreatic carcinoma group was higher than normal control group,there was statistically signficant difference（t=9.63,P 〈0.01）.Conculsion PTEN protein abnormally expressed and the changes of cell-cycle probably play an important role in pancreatic carcinoma.