高效液相色谱法测定芦荟配方颗粒中芦荟苷的含量

Content Detection of Aloin in Aloe Dispensing Granule by RP-HPLC

目的建立芦荟配方颗粒芦荟苷的高效液相色谱分析方法。方法 Shim-pack CLC-ODS-C18柱（6.0×150mm,10μm）;流动相：乙腈-水（25∶75）,流速：1.0m L·min^-1;柱温：35℃;检测波长355nm。结果芦荟苷在0.1612~1.6120μg范围内有良好的线性关系。平均回收率为98.50%（RSD=0.35%,n=6）。结论该方法简便快捷、准确、无干扰,可作为芦荟配方颗粒的内控质量标准。

OBJECTIVE To establish a reversed-phase high performance liquid chromatographic method for determination of aloin content in aloe dispensing granule. METHODS Shim-pack CLC-ODS-C18 column was used with the mobile phase being a mixture Acetonitrile-water（ 25∶ 75）,flow rate being 1. 0m L·min^-1,column temperature being 35℃,detecting wavelength being 355 nm. RESULTS The linear relationship of aloin from 0. 1612 ~1. 6120μg,The average recovery was 98. 50%（ RSD = 0. 35%,n = 6）. CONCLUSION The method is simple and quick、accurate、and no interference,and can be used for the quality control of aloe dispensing granule.