摘要
[目的]探讨microRNA-326(mi R-326)在脑胶质瘤中的表达及其临床意义。[方法]用qRT-PCR检测福尔马林固定、石蜡包埋(FFPE)的89例胶质瘤和20例正常脑组织病理标本。应用SPSS16.0分析miR-326的表达水平与临床病理学特征之间的相关性。[结果]脑胶质瘤(3.824±1.7011)和正常脑组织(6.135±1.2897)中mi R-326的表达水平有显著性差异(P<0.001)。miR-326的表达与肿瘤直径(P=0.029)、WHO分级(P<0.001)和KPS评分密切相关(P<0.001);与患者的年龄(P=0.518)和性别(P=0.501)无显著性相关。MiR-326低表达与胶质瘤患者的生存率降低密切相关(P<0.001)。胶质瘤的WHO分级增高、肿瘤直径>3cm、KPS评分<80分及miR-326低表达均是影响胶质瘤患者预后的危险因素(P<0.05)。[结论]miR-326在胶质瘤组织中呈低表达,其可能可成为预测胶质瘤发生发展及预后的生物标志物。
[Purpose] To investigate the expression and clinical significance of microRNA-326（miR-326） in gliomas. [Methods] The expression of miR-326 in 89 samples with glioma and 20 samples with normal brain tissue was detected by QRT-PCR,which were treated with formalin fixed,paraffin embedding. The correlation of miR-326 expression level and clinical pathological parameters was analyzed by SPSS16.0. [Results] The expression of miR-326 was significantly lower in glioma tissues（3.824±1.7011）than that in normal brain tissues（6.135±1.2897）（P〈0.001）.The expression of miR-326 was significantly correlated with tumor diameter（P=0.029）,WHO grade（P〈0.001）and KPS score（P〈0.001）,and did not correlate with age（P=0.518）and gender（P =0.501）. The lower expression of miR-326 was significantly associated with lower survival in patients with glioma. Tumor diameter greater than 3cm,KPS score less than 80 points,advanced WHO grade and low expression of miR-326 were the independent risk factors predicting poor prognosis for glioma patients（P〈0.05）. [Conclusions] miR-326 is down-regulated in glioma tissues.Mi R-326 might be a biomarker for predicting carcinogenesis and prognosis for glioma.
出处
《中国肿瘤》
CAS
2015年第11期957-960,共4页
China Cancer
