壮药战骨中柚皮素与芹菜素含量测定

Determination of Naringenin and Apigenin in Premna fulva Craib. by HPLC

目的：建立测定战骨中柚皮素和芹菜素含量的高效液相色谱法。方法以 SHISEIDO-SPOLAR C18柱（250 mm×4.6 mm,5μm）为色谱柱,流动相采用甲醇-0.2％磷酸梯度洗脱（42：58）,柚皮素的检测波长为288 nm,芹菜素的检测波长为340 nm,流速为1.0 mL·min^－1,柱温为35℃,进样量10μL。结果柚皮素在0.180-3.60μg 之间线性关系良好,r＝0.9999,芹菜素在0.0052-0.1040μg 之间线性关系良好,r＝0.9999。结论该方法准确、可靠,可用于战骨药材及相关制剂中柚皮素和芹菜素的含量测定。

Objective To establish a HPLC method for determination of naringenin and apigenin in Premna fulva. Methods The SHISEIDO-SPOLAR C18（250 mm×4.6 mm,5μm） was used as analytical column.The mobile phase consisted of methanol-0.2% phosphoric acid （42：58） with isocratic elution at a flow rate of 1.0 mL·min^-1 .The detection wavelength of naringenin and apigenin was 288 nm and 340 nm, respectively.Column temperature was set at 35 ℃ , the injection volumn was 10 μL. Results Naringenin and apigenin had a good linear relationship in the concentration range of 0.180 - 3.60 μg （r =0.999 9） and 0. 0052 - 0. 1040 μg （ r = 0. 999 9）, respectively. Conclusion The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin and apigenin in Premna fulva and its preparations.