断藤益母汤对佐剂性关节炎大鼠骨代谢标志物的影响

Effect of Duanteng Yimu Decoction on Bone Metabolism Markers of Adjuvantinduced Arthritis Rats

【目的】探讨断藤益母汤对大鼠骨代谢标志物血清I型胶原羧基末端肽(CTX-Ⅰ)、Ⅰ型前胶原氨基端前肽(PⅠNP)水平的影响。【方法】将72只SD大鼠分成9组,采用完全弗氏佐剂造模;给药36 d后,观察各组足肿胀度,血清CTX-Ⅰ、PⅠNP水平,及踝关节X线评分。【结果】各给药组足肿胀度、踝关节X线评分较模型组均显著降低(P<0.05),各组间血清PⅠNP水平比较差异均无统计学意义(P>0.05),复方低剂量组可显著降低血清CTX-Ⅰ水平(P<0.05)。【结论】南蛇藤对雌性佐剂性关节炎(AA)大鼠CTX-Ⅰ水平的作用呈双向,加入川续断、益母草组成复方后可降低南蛇藤副作用,随着南蛇藤剂量的提高,其对抗作用消失。

Objective To observe the effect of Duanteng Yimu decoction（DYD） on bone turnover markers of Cterminal telopeptide of type I collagen（CTX-Ⅰ） and N-terminal propeptide of type I procollagen（ P Ⅰ NP） of adjuvant-induced arthritis（AA） rats. Methods Seventy-two SD rats were divided into 9 groups, and complete Freund＇s adjuvant was injected into the rats to establish adjuvant-induced arthritis rat model. After medication for 36 days, the pedal swelling, the serum levels of CTX-Ⅰand PⅠNP, and ankle X-ray photographic scores were observed. Results The pedal swelling degrees and ankle X-ray scores in medication groups were lower than those in the model group（ P〈0.05）. The P Ⅰ NP level had no statistically significance in various groups（ P〉0.05）. The CTX-Ⅰlevel in low-dose DYD group was decreased（P〈0.05）. Conclusion Caulis Celastri has twoway effects on CTX-Ⅰlevel of female AA rats, and its side effects can be reduced by Radix Dipsaci and Herba Leonuri, but their antagonism effect will disappear with the increase of the dose of Caulis Celastri.