凉血化瘀方对MRL/lpr小鼠自身抗体及血清白细胞介素-6、17、21表达的影响

Effects of Blood-cooling and Blood-stasis-removing Decoction on Autoantibody and Serum Levels of Interleukins 6, 17, 21 in MRL/lpr Experimental Rats

【目的】观察中药复方凉血化瘀方对自发性系统性红斑狼疮(SLE)模型MRL/lpr小鼠抗双链DNA抗体(ds DNA)、抗核小体抗体(Anu A)及血清白细胞介素6(IL-6)、IL-17、IL-21表达的影响。【方法】实验小鼠分为空白对照组、模型组、中药高剂量组(剂量为25.2 g/kg)、中药低剂量组(剂量为12.6 g/kg),连续给药8周,眼眶取血并分离血清,采用酶联免疫吸附(ELISA)法检测抗ds DNA抗体、Anu A、IL-6、IL-17、IL-21水平。【结果】模型组小鼠血清自身抗体和IL-6、IL-17、IL-21水平较空白对照组显著升高(P<0.01),中药组血清抗体和细胞因子水平较模型组降低,其中中药高剂量组与模型组比较,差异均有统计学意义(P<0.01)。【结论】凉血化瘀方能降低自身抗体水平,抑制炎性细胞因子的表达,是其有效治疗SLE的机制之一。

Objective To observe the effect of blood-cooling and blood-stasis-removing Decoction on anti-double-strain DNA（ anti-ds DNA） antibody, anti-nucleosome antibody（ Anu A） and serum levels of interleukin（ IL）-6, 17, 21 in spontaneous systemic lupus erythematosus（ SLE） MRL/lpr experimental rats.Methods The experimental rats were divided into blank control group, model group, and high-and low-dose Chinese medicine groups（ 25.2, 12.6 g/kg respectively）, the treatment lasting 8 weeks. At the end of the experiment, the blood taken from the orbital veins was separated for obtaining serum, and then the serum anti-ds DNA antibody, Anu A, IL-6,17,21 levels were tested by enzyme-linked immunosorbent assay（ ELISA）.Results The serum autoantibody and IL-6, 17, 21 levels of rats in the model group were increased significantly as compared with the blank control group（ P〈0.01）. The serum antibody and cytokine levels of Chinese medicine groups were reduced as compared with the model group, the difference between high-dose Chinese medicine group and model group being significant（ P〈0.01）. Conclusion Blood-cooling and blood-stasis-removing Decoction has certain effect on reducing the serum levels of anti-ds DNA antibody, Anu A, and IL-6,17,21, which may coniribute to one of its therapeutic mechanisms for SLE.