健脾补血方对C57BL/6肺癌小鼠化疗后神经激肽1受体和CD34蛋白表达的影响

Effect of Jiangpi Buxue Decoction on Protein Expression of Neurokinin 1 Receptor and CD34 in C57BL/6 Mice with Lung Cancer After Chemotherapy

【目的】观察健脾补血方对C57BL/6肺癌小鼠化疗后神经激肽1受体(NK1R)和CD34蛋白表达的影响,探讨健脾补血方对化疗后恶心呕吐和骨髓抑制的改善作用。【方法】将40只C57BL/6小鼠正常饲养7 d后,右前肢腋下接种瘤细胞,随机分为单纯肺癌组、模型组和中药组。模型组与中药组均腹腔注射环磷酰胺1 d(剂量为80 mg/kg),单纯肺癌组与模型组灌服生理盐水,中药组以健脾补血方高、中、低剂量灌胃(剂量分别为25、12.5、6.25 g/kg),给药14 d,分别取脑、脾2个标本,采用Western blot法检测各组NK1R和CD34的蛋白表达水平。【结果】与单纯肺癌组比较,模型组脑组织中NK1R和脾组织中CD34的蛋白表达量均显著升高(P<0.01);与模型组比较,健脾补血方高、中、低剂量组脑组织中NK1R的蛋白表达量均显著下降(P<0.01),中、低剂量组脾组织中CD34的蛋白表达量显著升高(P<0.05)。【结论】健脾补血方能下调C57BL/6肺癌小鼠化疗后脑组织中NK1R的蛋白表达,并上调脾组织中CD34蛋白表达,提示健脾补血方可能具有抑制化疗后恶心呕吐及改善化疗后骨髓抑制的作用。

Objective To observe the effect of Jianpi Buxue Decoction on the protein expression of neurokinin 1receptor（NK1R） and CD34 in C57BL/6 mice of lung cancer after chemotherapy, and to explore the improvement of chemotherapy-induced nausea and vomiting and myelosuppression treated with Jianpi Buxue Decoction.Methods Forty C57BL/6 mice were transplanted with Lewis lung cancer cells in the armpit of left anterior limb after being fed for 7 days, and then were randomly divided into lung cancer group, model group, and high-,middle-and low-dose Chinese medicine groups. Model group and Chinese medicine groups were injected intraperitoneally with cyclophosphamide（80 mg/kg） for one day. Lung cancer group and model group were given normal saline. Chinese medicine groups were administered with high-, middle-and low-dose of Jianpi Buxue Decoction（25, 12.6, 6.25 g/kg respectively） for 14 days. After the modeling, all of the mice were sacrificed, and then the brains and spleens were sampled. Western blotting method was used to detect the protein expression of NK1 R and CD34. Results Compared with lung cancer group, the protein expression of cerebral NK1 R and splenic CD34 in the model group was increased significantly（ P〈0.01）. Compared with the model group, the protein expression of NK1 R in mice brain tissues of high-, middle-and low-dose Jianpi Buxue Decoction groups was decreased significantly（P〈0.01）, while the protein expression of CD34 in spleen tissues of middleand low-dose Jiangpi Buxue Decoction groups was increased obviously（ P〈0.05）. Conclusion Jianpi Buxue Decoction has an effect on down-regulating the protein expression of NK1 R in brain tissues and on up-regulating the protein expression of CD34 in spleen tissues of C57BL/6 mice with lung cancer after chemotherapy,indicating that Jianpi Buxue Decoction probably can relieve chemotherapy-induced nausea and vomiting, and can improve the myelosuppression after chemotherapy.