蒙药漏芦花质量标准研究

Quality Standard of Mongolian Medicine Rhaponticum uniflorum(L.) DC.

【目的】研究蒙药漏芦花的质量控制方法,为建立其药材质量标准奠定基础。【方法】采用薄层色谱方法鉴别漏芦花绿原酸、芹菜素;采用高效液相色谱（HPLC）分析方法,测定漏芦花中绿原酸成分的含量,其色谱柱：Kromat Universil C18（4.6 mm×250 mm,5μm）,流动相：甲醇—水—冰醋酸（体积比为15∶85∶0.85）,检测波长：326 nm,流速：1.0 m L/min,柱温：20℃。【结果】漏芦花薄层色谱供试品与对照品在相同的位置上显相同颜色斑点。绿原酸在0.044 66~0.446 6μg范围内呈良好的线性关系（r=0.999 8）,平均回收率为99.84%。【结论】所建立的方法简单、准确、重复性好,可用于蒙药漏芦花药材的质量控制。

Objective To establish the quality standard for mongolian medicine Rhaponticum uniflorum（L.） DC.with modern analysis methods. Methods Chlorogenic acid and apigenin were identified by thin-layer chromatography（TLC）. The chlorogenic acid content was determined by high performance liquid chromatograghy（ HPLC）. And HPLC was performed on Kromat Universil C18（ 4.6 mm × 250 mm, 5 μm） with methanol-water-acetic acid（v / v / v, 15 ： 85 ： 0.85） as mobile phase, the detection wavelength was 326 nm, the flow rate was 1.0 mL /min, and the column temperature was 20℃. Results The samples and the reference substance shared the same color spots at the same sites. The linear range of chlorogenic acid was 0.044 66-0.446 6 μg（r=0.999 8）,and the average recovery rate was 99.84%. Conclusion The method is simple, accurate, and with good reproducibility, and can be used for the quality control of Rhaponticum uniflorum（ L.） DC.