芝麻隐性细胞核雄性不育基因的特异SCAR标记

Development of specific SCAR markers identifying recessive genic male sterile gene in sesame( Sesamum indicum L. )

为了开发与芝麻隐性细胞核雄性不育基因连锁且利用简便的标记,以芝麻隐性细胞核雄性不育两用系95ms-5AB为材料,在前期应用AFLP标记进行遗传定位分析的基础上,开发出7个可鉴别该隐性细胞核雄性不育基因的SCAR标记。这些标记在可育池中可扩增出806bp^1 638bp的片段,而在不育池中没有扩增出相应的片段,将这些片段命名为PMC-1~PMC-7,在95ms-5AB的1 184株的大群体中检测到这些标记与不育基因间交换率为0.25%。利用这7个标记对来自95ms-5的4个不同遗传背景的F2不育株和可育株,以及来自2个不同遗传位点的不育系后代进行检测,表明它们可以特异地检测95ms-5不育基因。

To develop a simple and convenient marker for genetic linkage of sesame male sterile gene,based on an AFLP marker,7 SCAR（ sequence characterized amplified region） markers were developed using recessive genic male sterile two- type line 95ms- 5AB as fertile and sterile gene bulks. These 7 markers could detect bands from fertile bulk with 806 bp to 1 638 bp in size,and could not from sterile bulk. They were designated as PMC- 1to PMC- 7. These markers were used to detect 1 184 fertile and sterile plants of 95ms- 5AB with 0. 25% exchange rate. 4 F2 populations obtained from 95ms- 5A and different genotypes and 2 recessive genic male sterile lines were identified using these 7 SCAR markers. Result indicated that the SCAR markers were specific for 95ms- 5.