泽兰醇提物对实验性心肌缺血大鼠的保护作用研究

Proctective effects of alcohol extraction of eupatorium on experimental myocardial ischemia in rats

目的观察泽兰醇提物对实验性心肌缺血大鼠的保护作用。方法给大鼠灌服泽兰醇提物7天后,结扎冠状动脉左前降支(LAD),造成大鼠心肌缺血性损伤(AMI)模型。术后给药三天,观察心电图(EKG)、心肌梗死面积(MIS)和血清乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)、超氧化物歧化酶(SOD)、丙二醛(MDA)的变化。结果泽兰醇提物能改善AMI大鼠的EKG(P﹤0.01);泽兰醇提物800 mg·kg-1、400 mg·kg-1可缩小AMI大鼠梗塞面积,减少血清LDH漏出量,增强血清SOD活力,减少MDA生成(P﹤0.01,P﹤0.05);泽兰醇提物400 mg·kg-1能降低血清CK漏出量(P﹤0.05)。结论泽兰醇提物对心肌缺血性损伤有一定的保护作用。

Objective： To study the proctective effects of alcohol extraction of eupatorium on experimental myocardial is-chemia in rats. Methods： An animal model of acute myocardial ischemia injury （AMI） was constructed by ligating the leftanterior descending coronary artery（LAD） after alcohol extraction of eupatorium was given to the rats for 7 days. Three dayslater, the changes of Eleetrokardiogram （ EKG ）, myocardial infarction size （MIS） and lactic dehydrogenase （ LDH ）, creatinephosphokinase （CK） , superoxide dismutase （SOD） , malondialdehyde （MDA） in serum of rats were measured Results ： Al-cohol extraction of eupatorium could improve the EKG（ P 〈 0. 01 ） ;alcohol extraction of eupatorium （800 mg· kg-1,400mg· kg - t ） could minimise the MIS , reduce the leakage of LDH, increase the activity of SOD and decrease the production ofMDA（P 〈 0. 01 ,P 〈 0. 05） ;400 mg · kg-l alcohol extraction of eupatorium could reduce the leakage of CK（P 〈 0. 05） Conclusion： Alcohol extraction of eupatorium possesses a proctective effect against myocardial ischemia injury.