基于分子分类的石斛兰杂交结实性探讨

Study on Cross Fruitfulness of Ornamental Dendrobium Based on Molecular Classification

为了探讨石斛兰的杂交结实性,以42个主栽品种和12个原生种为研究对象,利用SRAP分子标记对其亲缘关系进行分析,并根据分析结果进行杂交配组。结果表明,在配组的杂交组合中,按组组合结实率和单个组合结实率排列:品种组内杂交组合>品种组间杂交组合>原生种之间杂交组合>品种与原生种间杂交组合,正反交结实率存在较大差异。说明在石斛兰的杂交中,亲缘关系较近的结实率高,较远的结实率低。本研究可为石斛兰育种杂交亲本选配提供理论依据。

In order to study cross fruitfulness of ornamental Dendrobium,popular commercial Dendrobium cultivars was used to analys the genetic relationship by SRAP. The results showed that among the hybrid combination, the fructification percentage of combination and single combination setting percentage was： intragroup hybridization〉 intergroup hybridization 〉native species hybridization 〉between native species and cultivars hybridization,there was obvious difference of setting percentage of reciprocal cross. In general,the result indicated that in the hybridization of Dendrobium,close genetic relationship has higher setting percentage,and distant relationship showed adverse. This result could provide the scientific basis for the parent selection of Dendrobium hybridization breeding.