种植环境对不同Wx基因型稻米RVA谱的影响

Environmental Influences on RVA Properties of Starches From Rice With Different Wx Genotypes

为了解种植环境对不同Wx基因型稻米淀粉黏滞性谱(RVA谱)的影响,选择5个携带有5类不同Wx等位基因的水稻单片段代换系,分别在4个生长环境下种植,对其稻米RVA谱进行测定分析,研究环境及环境与Wx基因型间互作对RVA特征值的效应。结果显示,不同Wx基因型水稻材料RVA特征值在不同种植环境下均存在显著性差异。其中,消减值、崩解值、最高黏度和热浆黏度的变异系数较大,分别达到45.53%、29.75%、26.68%和26.17%;起浆温度和峰值时间变异系数较小,只有2.50%和2.96%。均方差分析结果表明,不同RVA谱特征参数的主要变异来源为Wx基因型变异,环境及环境与Wx基因型间互作对稻米RVA特征值均有显著影响。其中,峰值时间以Wx基因型间变异最大,占总变异的97.01%;最高黏度的Wx基因型间变异最小,占总变异的57.51%。环境效应对RVA特征值影响方面,峰值时间、消减值和起浆温度受环境的影响较小,而最高黏度、热浆黏度和崩解值则比较容易到环境影响。Wx基因与环境互作对崩解值、消减值和最高黏度影响较大,而对峰值时间和起浆温度影响较小。本研究结果可为优质水稻品种选育与栽培过程中选择适合的Wx基因型材料提供借鉴和参考。

To understand the influence of growth environment on viscosity（ RVA） profile of starches from different Wx genotype rice,five rice single-segment substitution lines carrying different Wx alleles,were selected and planted in four cropping environments to study the effects of environment and Wx genotype × environment on RVA properties. The results showed that there were significant differences in rice RVA characteristics among the four different cropping sites.Among them,setback form peak viscosity（ SB_P）,breakdown（ BD）,peak viscosity（ PV）,and hotpaste viscosity（ HPV） showed high values of variation coefficient,which reached to 45. 53%,29. 75%,26. 68%,and 26. 17%,respectively. Variation coefficients of peak time（ Ptime） and pasting temperature（ Ptemp） were relatively low,and only2. 50% and 2. 96%,respectively. Analyses of variance showed that the Wx genotypic variation exerted the strongest influence on RVA parameters,and the environment and Wx genotype × environment effects both were significant for RVA characteristics. Among them,Ptimehad the highest genotypic variance which was 97. 01% of the total variation,and PV had the lowest value of genotypic variance accounting for 57. 51% of the total. With regard to the influence of environment on RVA properties,effect of growth environment was very small for Ptime,Ptemp,and SB_P parameters,while was relatively high for PV,HPV,and BD. Genotype × environment interactions had relatively high effects on BD,SB_P,and PV,while had relatively low influence on Ptimeand Ptemp. The present results have important implications for rice breeders and producers to select favorite Wx alleles in rice grain quality improvement.