NaCl胁迫下不同种源沙枣的生理特性

Physiological Characteristics of Different Elaeagnus angustifolia L.Provenances Under NaCl Stress

为了解沙枣的耐盐生理机制,以不同种源沙枣实生苗(阿拉尔种源和银川种源)为材料,采用盆栽控盐试验,研究不同Na Cl浓度(0、0.15、0.30 mol·L-1)和不同胁迫时间(7、30、60 d)对沙枣生物量、根冠比及耐盐生理指标变化的影响。结果表明:随着Na Cl浓度增加和胁迫时间的延长,生物积累量和叶绿素含量逐渐减少。胁迫30 d后,阿拉尔种源生物积累量和叶绿素的最大下降幅度分别为36.92%和14.65%,而银川种源分别为41.86%和37.07%;随Na Cl浓度增加,根冠比和丙二醛含量逐渐增加,阿拉尔种源增加幅度小于银川种源。脯氨酸含量随着Na Cl浓度增加而增加,但随胁迫时间延长,增加幅度逐渐减少。胁迫7 d时,可溶性糖含量随盐浓度增加呈下降趋势,随胁迫时间延长,逐渐转换为增加,在胁迫60 d后,0.30 mol·L-1Na Cl水平下,阿拉尔沙枣叶片可溶性糖含量增加了36.69%,而银川仅有7.27%。因此,阿拉尔种源沙枣幼苗耐盐能力要强于银川种源,耐盐能力较强的沙枣种源可以更好的保护叶绿素,维持膜系统稳定,具有更强的渗透调节能力,保证生物量的积累。本研究可为优良耐盐沙枣种质筛选提供理论依据。

In order to further understand the physiological mechanism of Elaeagnus angustifolia L. salt trolerance,pot experiments were carried out to study effects of NaCl stress（ NaCl concentration of 0,0. 15 and 0. 30 mol·L^- 1） and stress time（ 7,30 and 60 days） on the biomass,root / shoot ratio and phsiological characteristics of different provenances of E.angustifolia L.（ Alaer provenance and Yinchuan provenance）. Results showed that biomass accumulation and chlorophyll content were gradually reduced with increasing NaCl concentration and stress time. After treated with salt for30 days,the largest decrement of the biomass accumulation and chlorophyll content in leaves of Alaer E. angustifolia L.provenance was 36. 92% and 14. 65%, respectively, and 41. 86% and 37. 07% for Yinchuan provenance,respectively. With increasing of NaCl concentration,root / shoot ratio and MDA content increase gradully,and Alaer provenance had a lower increasement than that of Yinchuan provenance. Content of proline was significantly increased with increase of the NaCl concentration,but the creased rate of proline content decreased with extention of the stress time. At 7 days after salt treatment,soluble sugar content of E. angustifolia L. decreased with the NaCl concentration incraseing. With the extension of stress time,the soluble surgar content gradully converted to increase with increase of the salt stress. The soluble sugar content of Alaer provenance was increased by 36. 69% under the 0. 3 mol·L^- 1for 60 days,while Yinchuan provenance was only 7. 27%. Therefore,the salt-tolerance of E. angustifolia L. seedlings from Alaer provenance is stronger than that from Yinchuan provenance. Salt-tolerant E. angustifolia L. provenance could better protect the chorophyll,maintain stability of the membrane system,and ability of osmotic regrlation,to ensure the biomass accumulation than salt-sensitive provenance. This provides a theoretical basis for screening excellent salttolerant E. angustifolia L. germplasm.