骨髓间充质干细胞在氨气致大鼠吸入性肺损伤的疗效观察

Efficacy Observation of Bone Marrow Mesenchymal Stem Cells in Ammonia Inhalation Induced Lung Injury

目的:观察骨髓间充质干细胞在氨气致大鼠吸入性肺损伤的疗效。方法:随机选取69只普通SD大鼠,随机将这些大鼠分为正常组(n=23)、地塞米松治疗组(n=23)、干细胞治疗组三组(n=23),正常组给予尾静脉注射生理盐水,地塞米松治疗组给予注射地塞米松,干细胞治疗组给予干细胞注射。结果:干细胞治疗组大鼠治疗后1 d、3 d的肺组织病理学评分及肺W/D、BALF白细胞数量及蛋白浓度、TNF-α、IL-8水平均显著低于地塞米松治疗组(P<0.05),均显著高于正常组(P<0.05)。结论:骨髓间充质干细胞在氨气致大鼠吸入性肺损伤的疗效较地塞米松显著。

Objective： To obseP〈0 marrow mesenchymal stem cells in ammonia inhalation induced lung injury.Methods： 69 cases of normal SD rats were randomly selected, the rats were randomly divided into normal group（n=23）, dexamethasone treatment group（n=23）, stem cells treatment group（n=23） three groups, the normal group were given tail vein injection of saline, dexamethasone treatment group were given injections of dexamethasone, stem cell therapy group were given injections of stem cells. Results：The lung histopathology scores and lung W / D, BALF white blood cell count and protein concentration, TNF-α, IL-8 1d, 3d after treatment of the stem cell treatment group were significantly lower than the dexamethasone treatment group（P〈0.05）, were significantly higher than the normal group（P〈0.05）. Conclusion： The efficacy of bone marrow mesenchymal stem cells in ammonia inhalation induced lung injury is significant than Dexamethasone.