胶原酶与自体血建立脑出血模型的比较研究

Comparative study on the model of intracerebral hemorrhage of collagenase and autologous blood establishment

目的比较胶原酶脑出血模型与自体血脑出血模型的稳定性。方法分别采用胶原酶和自体血注入大鼠尾状核建立脑出血模型,比较两组模型死亡率、神经功能缺陷评分、脑组织水含量、血肿大小、神经元细胞凋亡的差异。结果假手术组术后神经功能缺陷评分、脑水含量、血肿体积、神经元细胞凋亡数量未见明显变化;胶原酶组成功率高于自体血组(P<0.05);两组死亡率基本一致。胶原酶模型组24 h、3 d、7 d时间点神经功能缺陷、脑水含量、血肿体积、神经元细胞凋亡数量均明显高于自体血模型组(P<0.05)。结论胶原酶脑出血模型较自体血脑出血模型稳定。

Objective To compare the stability of collagenase model and autologous blood cerebral hemorrhage. Meth-ods The model of intracerebral hemorrhage(ICH)was established by collagenase and autologous blood into caudate nu-cleus in rats. The model mortality,neurological deficit score,brain water content,the size of hematoma and the number of neuronal apoptosis were choosed to detect the difference of two models. Results No significant difference of the neurologi-cal deficit score,brain water content,the size of hematoma and the number of neuronal apoptosis after operation in sham op-eration group;The model success rate in collagenase group was obviously higher than the autologous blood group( P <0. 05);The model mortality had no difference in two groups. At 24 h,3 d,7 d times,the neurological deficit score,brain wa-ter content,the size of hematoma and the number of neuronal apoptosisn of collagenase group were all higher than the autolo-gous blood group(P < 0. 05). Conclusion The ICH model of rats established by collagenase was a stable model.