拜颤停复方对帕金森病模型小鼠神经炎症的影响

Effects of Baichanting Compound on Neuroinflammatory Responses in Mice Models with Parkinson's Disease

目的探讨拜颤停复方对帕金森病(PD)模型小鼠神经保护作用的免疫炎症机制。方法腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶30 mg/(kg·d),连续5 d,诱导雄性C57BL/6小鼠制作PD模型。将实验小鼠随机分为正常组、模型组及拜颤停复方组,造模后第6日治疗组给予拜颤停复方181.50 mg/(kg·d)灌胃,连续20 d。通过爬杆实验及自主活动实验进行小鼠神经行为学观察,应用UPLC-MS-MS三重四级杆串联质谱仪检测小鼠纹状体多巴胺(DA)含量,ELISA检测中脑黑质部位炎症因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)及一氧化氮(NO)的水平。结果与正常组比较,模型组小鼠爬杆时间显著延长、自主活动次数及纹状体DA含量显著降低,IL-1β、IL-6、TNF-α、IFN-γ及NO水平均显著升高;与模型组比较,拜颤停复方组可显著缩短爬杆时间、增加自主活动次数及纹状体DA含量,显著降低各炎症因子及NO水平(P<0.05,P<0.01)。结论拜颤停复方可明显改善PD小鼠的神经行为学变化,降低小鼠中脑黑质炎症因子及NO水平,保护神经元。

Objective To discuss the immune inflammation mechanism of neuroprotective potential of Baichanting Compound on mice models with Parkinson's disease(PD). Methods The PD model mice were induced by intraperitoneal injecting MPTP 30 mg/(kg·d) for 5 days in male C57BL/6 mice. The mice were randomly divided into control group, model group and Baichanting Compound group. From the sixth day after modeling, Baichanting Compound group was given Baichanting Compound 181.50 mg/(kg·d) orally for gavage for 20 days. The pole and independent activity experiments were used to observe the neuroethology of mice. DA content in corpus striatum was detected by UPLC-MS-MS. The levels of inflammation factors, such as IL-1β, IL-6, TNF-α, IFN-γ and NO in midbrain nigra tissues were detected by ELISA. Results Compared with the control group, the time of climbing pole was significantly prolonged;the number of independent activities and DA content in corpus striatum decreased significantly;the levels of IL-1β, IL-6, TNF-α, IFN-γ and NO significantly increased in PD mice in the model group. Compared with model group, the time of climbing pole was shortened obviously;the number of independent activities and DA content in corpus striatum increased significantly;the levels of IL-1β, IL-6, TNF-α, IFN-γ and NO decreased significantly in PD mice in the Baichanting Compound group. Conclusion Baichanting Compound can improve neurobehavioral changes in PD mice obviously by reducing inflammation factors and NO level in substantia nigra to protect neurons.