荆芥挥发油的提取及胶体磨包合工艺研究

Extraction Technology of Volatile Oil from Schizonepetae Herba and Inclusion Technology by Colloid Mill

目的优选荆芥挥发油的提取及β-环糊精胶体磨包合工艺。方法采用水蒸馏法进行提取,以浸泡与否、加水倍量、蒸馏时间为考察因素,挥发油体积为评价指标,考察荆芥挥发油的提取工艺;以β-环糊精与挥发油比例、加水倍量、研磨时间为考察因素,挥发油包合率为评价指标,采用胶体磨研磨法,正交试验优选包合工艺。采用紫外分光光度法和显微成像分析对包合物进行验证。结果优选的提取工艺为荆芥加8倍量水,提取6 h;包合工艺为挥发油与β-环糊精比例为1∶8(m L∶g),加8倍量水,研磨30 min。结论所选工艺合理、可行,可用于提取荆芥中挥发油及挥发油与β-环糊精的包合。

Objective To optimize the extraction technology of volatile oil from Schizonepetae Herba and the inclusion technology by colloid mill of β-cyclodextrin. Methods Water distillation method was used to extract. Infusion or not, amount of water and distillation time were set as factors, and the volume of volatile was set as indexes to examine the extraction technology of volatile oil from Schizonepetae Herba;the ratio of β-cyclodextrin to volatile oil, inclusionn time, amount of water were set as factors, and the yield of inclusion compounds and inclusion rate of volatile oil were set as indexes to use colloid mill grinding method and orthogonal test to optimize the inclusion technology. Ultraviolet spectrophotometry and microscopic imaging analysis were used to verify this inclusion compound. Results The optimized extraction conditions were:adding 8-fold water, extracting for 6 h without infusion. The optimized inclusion conditions were:the proportion of volatile oil to β-cyclodextrin=1∶8(m L∶g), adding 8-fold water, milling for 30 min. Conclusion The technology is reasonable and feasible, which can be used for the extraction of volatile oil from Schizonepetae Herba and the inclusion of β-cyclodextrin.