HPLC测定当归5种化学成分不确定度评定

Evaluation of Uncertainty Measurement on 5 Kinds of Chemical Substance in Angelicae Sinensis Radix by HPLC

目的建立高效液相色谱法(HPLC)同时测定当归中阿魏酸、正丁基苯酞、正丁烯基苯酞、Z-藁本内酯及亚油酸的不确定度评定方法。方法采用HPLC同时测定当归中5种化学成分,分析检测过程中的各种不确定因素,并对各影响因素进行考察。结果用HPLC测定当归中阿魏酸、正丁基苯酞、正丁烯基苯酞、Z-藁本内酯和亚油酸的合成标准不确定度分别为3.23×10-2、4.59×10-2、1.19×10-2、2.15×10-2和8.72×10-2,即当归中5种化学成分在测定中的不确定度大小依次为亚油酸>正丁基苯酞>阿魏酸>Z-藁本内酯>正丁烯基苯酞。结论不确定度评定可为当归有效成分的定量研究提供一定参考。

Objective To establish a method for evaluating uncertainty of determining ferulic acid, 3-butylphthalide, Z-butylidenephthalide, Z-ligustilide and linolic acid in Angelicae Sinensis Radix by HPLC. Methods HPLC was used for simultaneously determining the 5 kinds of chemical substance in Angelicae Sinensis Radix, and various uncertain factors and these factors in the process of measurement were analyzed and investigated. Results The synthetical standard uncertainty of ferulic acid, 3-butylphthalide, Z-butylidenephthalide, Z-ligustilide and linolic acid in Angelicae Sinensis Radix measured by HPLC were 3.23×10-2, 4.59×10-2, 1.19×10-2, 2.15×10-2and 8.72×10-2, respectively. The uncertainty of 5 kinds of chemical substance in Angelicae Sinensis Radix was linolic acid > 3-butylphthalide > ferulic acid > Z-ligustilide >Z-butylidenephthalide. Conclusion The evaluation of uncertainty measurement can be used to offer certain references for quantitative research on active ingredients in Angelicae Sinensis Radix.