斑马鱼消化器官发育缺陷突变体的遗传筛选

Genetic screening of zebrafish mutants with developmental defects in the digestive organs

目的正向遗传筛选斑马鱼肝脏、肠和胆囊发育缺陷突变体。方法 ENU诱变野生型斑马鱼并开展经典的F2代筛选,以lfabp为探针的全胚原位杂交、BES-H2O2-Ac荧光染料分别检测斑马鱼早期胚胎肝脏、肠和胆囊的表型。结果在128个突变基因组中筛选获得了源自14个F2家族的斑马鱼消化器官发育缺陷突变体品系23个,并按表型划分为6类。结论斑马鱼肝脏、肠和胆囊的发育调控机制有相似性和差异性。

Objective To obtain zebrafish mutants with developmental defects in digestive organs from a forward genetic screening .Methods ENU mutagenesis and a classical F 2 genetic screening were performed .RNA whole mount in-situ hybridization using lfabpas probe and BES-H2 O2-Ac staining was applied to examine the liver , intestine and gall blad-der phenotype in zebrafish embryos .Results We harvested 23 mutant lines with developmental defects in digestive organs （originated from 14 F2 families） after screening 128 mutagenized genomes.These mutants were classified into six groups according to their phenotypes .Conclusions The liver, intestine and gall bladder share and differ in their developmental molecular mechanisms in zebrafish .