雌雄激素联合作用对高脂血症小鼠血脂水平及凝血功能的影响

The synergistic effect of estradiol and testosterone on the lipids and coagulation function in mice with hyperlipidemia

目的探讨雌雄激素联合作用对高脂血症小鼠血脂水平及凝血功能的影响。方法对小鼠行去势手术后通过高脂饮食建立高脂血症模型,通过给予不同激素将老鼠分为5组。造模结束后采集小鼠血液,一部分小鼠分离血清检测小鼠血脂水平,一部分小鼠分离血浆检测凝血功能。结果高脂饮食后,小鼠血清总胆固醇、甘油三酯和低密度脂蛋白水平较对照组相比明显升高,凝血酶原时间、活化部分凝血酶原时间时间明显缩短(P<0.05),给予雌雄激素联合治疗后,与造模组相比,小鼠血清总胆固醇、甘油三酯和低密度脂蛋白水平较对照组相比明显下降,凝血酶原时间、活化部分凝血酶原时间时间明显延长(P<0.05),而与对照组无明显差异。结论雌雄激素联合治疗可以调节血脂,显著降低低密度脂蛋白的水平,调节凝血功能,提示雌雄激素联合作用可以降低冠心病的发病风险,为冠心病的激素治疗提供新思路。

Objective To investigate the influence of synergistic effect of estradiol and testosterone on the level of lipids and coagulation function in mice with hyperlipidemia. Methods We established a maouse model in hyperlipidmia,giving estradiol（ 1 μg / d）, testosterone（ 7 μg / d） or estradiol combined testosterone（ 1 μg / d E2 ＋ 7Tμg / d）,respectively. After 14 weeks,we collected the blood from the mice,separated the serum to detect the lipids level,separated the plasma to test the coagulation function. Result Compared with controls,after high-fat diet,the serum level of total cholesterol（ TC）,triglyceride（ TG）,high density lipoprotein（ HDL） were significantly increased,PT and APTT were shorter（ P〈0. 05）. However,after treating with estradiol combined testosterone,compared with cases,the serum level of C,TG and HDL were lower and PT,APTT were longer（ P〈0. 05）,had no significance with controls.Conclusion The synergistic effect of estradiol and testosterone can mediate the lipids,reduce the level of LDL,regulate the coagulation function,reduce the risk of coronary heart disease,and also provide a new strategy for hormone therapy in coronary heart disease.