天南星总黄酮纳米凝胶对Walker256骨癌痛模型大鼠的镇痛作用研究

Experimental study of the analgesic mechanism of total flavonoids of Rhizoma Arisaematis nanogel on Walker256 induced bone cancer pain

目的研究天南星总黄酮纳米凝胶外用对Walker256骨癌痛大鼠的镇痛作用及其对骨结构的影响。方法将雌性SD大鼠随机分为空白组、假手术组、天南星组、唑来膦酸组,每组9只。除假手术组外,其余各组采用Walker256大鼠乳腺癌细胞制备大鼠胫骨癌痛的骨癌模型。造模后各组采取相应的干预措施。观察大鼠的机械痛、热痛觉敏感变化及四足承重变化,并取大鼠患侧胫骨行HE染色及Micro CT检测骨容积、骨小梁数目及厚度、骨皮质厚度。结果 Walker256大鼠乳腺癌细胞制备大鼠胫骨癌痛的模型成功。天南星组、唑来膦酸组机械痛阈值与空白组比较差异无统计学意义(P>0.05)。唑来膦酸组、天南星组大鼠热痛敏感时间均较空白组延长(P<0.05)。天南星组、唑来膦酸组右后肢承重比值与空白组相比明显增加(P<0.05);天南星组骨小梁数目较空白组增多(P<0.05),但天南星组骨小梁数目较唑来膦酸组减少(P<0.05)。结论天南星总黄酮纳米凝胶有一定的镇痛作用及护骨作用。

Objective This study designed to observe the analgesic effect of total flavonoids of Rhizoma Arisaematis nanogel on Walker256 induced bone cancer pain model,and explore the analgesic mechanism. Methods Female SD rats were randomly divided into five groups： blank group, sham operation group, Rhizoma Arisaematis group and zoledronic acid group, 9 in each group. Series of indexes were observed including mechanical thresholds,thermal thresholds,dynamic weight bearing ability together with bone HE stain and bone radiology. Results Indicated that rat model of hyperalgesia and allodynia was achieved after intra-tibial injectiun of Walker 256 cells. The thermal thresholds and dynamic weight bearing ability of control rats and bone caner rats were significant different （ P 〈 0.05）. However difference of mechanical thresholds between control rats and bone cancer rats was not remarkable （ P 〉 0.05 ）. Furthermore, Trabecular number in Rhizoma Arisaematis group and zoledronic acid group was highly increased compared with the control group. Conclusion Total flavonoids of Rhizuma Arisaematis nanogel may have the analgesic effect in the development of bone cancer,and this finding might provide a new analgesic strategy tot reducing cancer pain.