微型大白菜“娃娃菜”游离小孢子出胚率影响因素研究

Effect of Frequency of Embryos on Microspore Culture in Miniature Chinese Cabbage 'Baby cabbage'

为了创制新、奇、特大白菜优异DH系,丰富大白菜种质资源,试验以3个"娃娃菜"优良杂交种为试材进行小孢子培养,通过优化培养条件建立高效培养体系,对影响小孢子胚发生的主要因素研究,结果表明:不同基因型间的小孢子胚诱导率差异极显著;4℃低温预处理24 h对小孢子胚的发生具有促进作用;33℃条件热击48 h,胚诱导率达最大值;NLN-13+0.05 mg/L 6-BA+0.05 mg/L NAA为适宜的诱胚培养基。

This study is to develop new,odd,excellent DH lines in Chinese Cabbage for rich in Germplasm Resources.Isolated microspore culture was employed in 7 hybrid varieties.An efficient culture system was es-tablished through the optimization of culture conditions.Results showed that embryoids and 335 regenerated plants were obtained.Genotypes had extremely significant effect on frequency of embryogenesis.Low tempera-ture pretreatment at 4 ℃ for 24 h couldenhance embryo frequencies.Temperature pretreatment at 33 ℃ for 48 h couldenhance embryo frequencies.NLN-13 ＋0.05 mg /L 6-BA ＋0.05 mg /L NAA could improve the fre-quency ofmicrospore embryogenesis.