摘要
宫颈癌的发病率在女性恶性肿瘤中占第二位。miR-124的表达在宫颈癌组织中显著下调。目前,发现在宫颈癌细胞中高表达miR-124可显著抑制宫颈癌细胞的生长、迁移与侵袭能力。此外,miR-124在宫颈癌细胞中的高表达还可显著抑制Cdcp1基因的表达。Luciferase实验结果表明Cdcp1是miR-124的直接下游靶标基因。在转染miR-124的宫颈癌细胞中高表达CDCP1蛋白可抵消由于miR-124引起的肿瘤抑制作用。这对揭示microRNA在宫颈癌发生、发展中的作用机制及基于microRNA的宫颈癌临床治疗具有一定的潜在意义。
Cervical cancer is the second most common malignant tumor in women around the world. The expression of miR-124 is significantly down-regulated in cervical cancer. The results showed that exogenous expression of miR-124 could remarkably suppress the proliferation and migration of cervical cancer cells. Luciferase analysis showed that CUB domain containing protein 1 gene （ Cdcpl ） is a direct down-stream target of miR-124 and restoration of CDCP1 counteracted the tumor suppressive effects of miR-124. The results have a great potential for not only the understanding of the relationship between miRNA and cervical cancer, but also microRNA-based anti-cervical cancer therapy.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2015年第10期13-19,共7页
China Biotechnology
基金
国家自然科学基金资助项目(31440059)
