摘要
目的基于观察p38MAPK信号通路相关指标的变化,探讨sCD40L及防御素-7在急性重症胰腺炎(severe acute pancreatitis,SAP)中的作用。方法 45只雄性SD大鼠随机分为假手术组(SO组)、SAP对照组(SAP组)及SAP-CNI1493干预组(SAPCNI1493组)。采用50 g/L牛磺胆酸钠胰胆管逆行注射建立SAP模型,术后1、3、6 h处死大鼠并取血。对胰腺进行病理组织学评分。RT-PCR法检测大鼠胰腺组织p38MAPK mRNA及回肠末端组织防御素-7 mRNA表达;ELISA方法检测血清sCD40L水平;光镜下评估胰腺组织病理学积分。结果 SAP组各时间点p38MAPK、sCD40L及胰腺组织病理学积分较SAP-CNI1493组明显升高(P<0.05);SAP组各时间点防御素-7表达水平较SAP-CNI1493组明显下降(P<0.05)。结论 p38MAPK信号通路介导sCD40L及防御素-7表达在SAP病情发生及发展中占据重要地位。
Objective To investigate the effects of sCD40L and defensin-7 on severe acute pancreatitis （SAP） based on p38MAPK signaling pathway. Methods Forty-five male SD rats were randomly divided into SO group, SAP group and SAP-CNI1493. The SAP model was established by injecting the 50 g/L sodium taurocholate. All rats were sacrificed and taken blood after 1, 3, 6 hours. Histopathological score of pancreas had done and ascites volume was measured in rats. The expression of p38MAPK mRNA in pancreatic tissue and the defensin-7 mRNA in terminal ileum tissue were detected by RT-PCR. The serum level of sCD40L was detected by ELISA. The pathological integral of pancreatic tissue was evaluated under the light microscope. Results The levels of p38MAPK, sCD40L, volume of ascites and pancreatic histopathology integral were significantly increased in SAP group compared with SAP-CNI1493 group （P 〈 0.05）. The expression of defensin-7 was significantly decreased in SAP group compared with SAP-CNI1493 group （P 〈 0.05）. Conclusion The sCD40L and defensin-7 based on the p38MAPK signal pathway play important roles in the occurance and development of SAP.
出处
《胃肠病学和肝病学杂志》
CAS
2015年第11期1365-1368,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
广西中医药管理局项目(GZZC14-30)
