养心草安神颗粒镇静催眠药效及急性毒性研究

Study on the Sedative-hypnotic Effect and Acute Toxicity of Yangxincao Anshen Granule

目的:考察养心草安神颗粒安神药效及急性毒性。方法:通过观察小鼠自主活动及协同戊巴比妥钠睡眠实验考察养心草安神颗粒镇静催眠的效果,运用最大给药量法给予养心草安神颗粒浸膏,连续观察14 d,同时记录小鼠毒副反应情况。结果:养心草安神颗粒高、中剂量均能明显减少小鼠自主活动次数、增加小鼠睡眠只数和延长小鼠睡眠时间(P<0.05)。急毒试验表明,动物未见任何急性毒性反应,测得养心草安神颗粒浸膏的最大给药量为306.6 g生药/kg,相当于成人一日用量的408.8倍。结论:养心草安神颗粒具有显著地镇静催眠作用,同时毒性较小,临床应用安全可靠。

Objective：To study the sedative-hypnotic effect of Yangxincao Anshen Granule and its acute toxicity. Methods：The sedative-hypnotic effect was evaluated by the spontaneous activity and sodium pentobarbital-induced sleep in mice model. According to maximum dosage test,the acute toxicity reaction of mice with intragastrical administration of water extract of Yangxincao Anshen Granule was recorded in the successive 14 days. Results：The results showed that Yangxincao Anshen Granule could significantly decrease the spontaneous activity of mice（P〈0.01）,prolong sleep time（P〈0.05）and increase rates of sleeping in mice（P〈0.05）on the high and medium dosages compared with the control group. Moreover,there was no acute toxic reaction during the acute toxic test. The maximum dosage was 306.6 g crude drug/kg in mice,which was 408.8 times adult daily dosage. Conclusion：Yangxincao AnshenGranule possessed significant sedative-hypnotic effect and no obvious toxicity.