栀子苷对佐剂性关节炎大鼠滑膜细胞中IL-17水平影响

Investigate Geniposide Il-17 Levels in Rats with Adjuvant Arthritis Synovial Cells

目的:探讨栀子苷对佐剂性关节炎大鼠滑膜细胞中IL-17水平的影响,为认识类风湿性关节炎的发病机制和临床治疗类风湿性关节炎提供实验依据。方法:用弗氏完全佐剂制备佐剂性关节炎模型,造模成功取滑膜细胞进行培养,体外给一定剂量的栀子苷,并用酶联免疫吸附法测定IL-17水平的变化。结果:AA大鼠造模成功后,以酶联免疫法检测大鼠滑膜细胞中IL-17的含量。模型组大鼠IL-17水平明显升高,与空白对照组比较差异有统计学意义(P<0.01)。与模型组比较,各给药组均能显著降低IL-17水平(P<0.01)。结论:栀子苷有显著降低AA大鼠滑膜细胞中IL-17水平的作用,提示栀子苷对佐剂性关节炎治疗作用可能与抑制炎性细胞因子IL-17有关。

Objective：To investigate Geniposide IL-17 levels in rats with adjuvant arthritis synovial cells,to provide experimental basis for the understanding of the pathogenesis and clinical treatment of rheumatoid arthritis in rheumatoid arthritis. Methods：Freund＇s complete adjuvant preparation adjuvant arthritis model,a successful modeling synovial cells were cultured in vitro to a certain dose of Geniposide,determination of changes in the level of IL-17 and then the enzyme-linked immunosorbent assay. Rusults：AA rat model was successfully detected by ELISA rat IL-17 content. The model rats IL-17 levels were significantly increased compared with the control group,the difference was statistically significant（P〈0.01）. Compared with model group,all the medicated group significantly reduced IL-17 levels（P〈0.01）. Conclusion：Geniposide significantly reduce the AA rats synovial cells in IL-17 levels,suggesting that the Geniposide adjuvant arthritis role may inhibit inflammatory cytokines IL-17 related.