黄芪多糖配伍三七总皂苷对糖尿病模型大鼠肾脏保护作用研究

Effect of Astragalus Polysaccharides Combined with Panax Notoginseng Saponins on Kidney of Rats with Diabetic Nephropathy

目的探讨黄芪多糖与三七总皂苷配伍对糖尿病大鼠肾脏功能的保护作用。方法 SPF级SD大鼠60只,10只为正常对照组,50只予腹腔注射链脲佐菌素建立糖尿病大鼠模型,将糖尿病模型大鼠随机分为模型组、黄芪多糖组、三七总皂苷组、黄芪多糖与三七总皂苷配伍组、贝那普利组,每天给相应药物1次,连续8周。8周后测定各组大鼠24h尿总蛋白、血糖、血肌酐和尿素氮;取肾脏进行病理组织学检查。结果与正常对照组比较,模型组大鼠24h尿总蛋白[(103.76±4.67)mg/24h比(13.82±4.49)mg/24h]、血糖[(28.44±3.74)mmol/L比(6.59±1.11)mmol/h]、血肌酐[(111.91±9.46)μmol/L比(44.78±4.12)μmol/L]和尿素氮[(10.90±1.76)mmol/h比(5.53±1.27)mmol/L]显著升高(P<0.01);病理组织学检查可见模型组大鼠肾小球基底膜增厚,肾小管出现大量炎症细胞浸润,间质纤维化;与模型组比较,黄芪多糖组大鼠尿蛋白[(84.01±7.20)mg/24h]、血糖[(20.60±2.10)mmol/L]和肌酐[(80.58±4.52)μmol/L]明显下降(P<0.05);三七总皂苷组大鼠血糖[(21.86±4.08)mmol/L]和肌酐[(77.79±5.59)μmol/L]下降(P<0.05);黄芪多糖和三七总皂苷配伍组大鼠尿蛋白[(66.00±3.43)mg/24h]、血糖[(16.34±2.34)mmol/L]、肌酐[(68.94±3.22)μmol/L]和尿素氮[(7.43±1.48)mmol/L]含量均明显下降(P<0.01)。各给药组肾脏病理改变均有不同程度改善。结论黄芪多糖与三七总皂苷配伍对糖尿病大鼠的肾脏具有保护作用,其作用优于黄芪多糖和三七总皂苷单用组。

Objective To investigate effect of Astragalus Polysaccharides（APS） combined with Panax Notoginseng saponins（PNS） on kidney of rats with diabetic nephropathy. Methods Diabetic nephropathy was induced by intraperitoneal injection of streptozotocin. A total of 50 diabetic rats were randomlydivided into model group, APS group, PNS group, PNS combined with APS group, benazeprilgroup. Rats were administered the corresponding drug intragastrically dailyfor 8 weeks. Urinary protein level in 24 h, blood sugar, blood creatinine, and urea nitrogen level and histopathological examination of the kidney were determined.Another 10 rats served as normal control. Results Urinary protein level in 24 h, blood glucose, creatinine, and urea nitrogen level in rats in model group were significantly higher than those in normal control group（103.76±4.67mg/24 h vs 13.82 ±4.49mg/24 g, 28.44 ±3.74mmol/L vs6.59 ±1.11mmol/L, 111.91 ±9.46μmol/L vs 44.78 ±4.12μmol/L, and 10.90 ±1.76mmol/h vs 5.53 ±1.27mmol/L; all P 〈0.05）. Histopathological examination showed glomerular basement membrane thickening, a large number of inflammatory cell infiltration into tubular, and interstitial fibrosis. Compared with model group, APS reduced urinary protein to 84.01±7.20mg/24 h, blood glucose to 20.60±2.10mmol/L, and creatinine content to 80.58±4.52μmol/L（all P 〈0.05）; PNS decreased blood glucose to 21.86±4.08mmol/L and creatinine content to 77.79±5.59μmol/L（all P 〈0.05）;in compatibility groupurinary protein, blood glucose, creatinine, and urea nitrogen content were further lowedto66.00 ±3.43mg/24 h, 16.34 ±2.34mmol/L, 68.94 ±3.22μmol/L, and 7.43 ±1.48mmol/L（all P 〈0.05）. Renal pathological changes had greatly improved in treatment groups. Conclusion APS combined with PNS haveprotective effect on kidney of rats with diabetic nephropathy, and the effect was better than that of APS and PNS alone.