红树尖瓣海莲内生真菌Phomopsis longicolla HL-2232次级代谢产物研究

Secondary Metabolites of a Bruguiera sexangula var. RhynchopetalaDerived Fungus Phomopsis longicolla HL-2232

通过活性追踪的方法,从一株来源于药用红树尖瓣海莲的内生真菌Phomopsis longicolla HL-2232中分离鉴定了5个生物碱类化合物、1个色原酮类化合物以及4个甾醇类化合物,分别鉴定为：6-氨基嘌呤-9-羧酸甲酯（1）,腺嘌呤核苷（2）,尿嘧啶核苷（3）,N,N＇-二苯基尿素（4）,（2S,2＇R,3R,4E,8E,3＇E）-2-（2＇-羟基-3＇-十八碳烯酰胺）-9-甲基-4,8-十八碳二烯-1,3-二醇（5）,2-（2＇S-羟丙基）-5-甲基-7-羟基对氧萘酮（6）,fortisterol（7）,（22E）-5α,8α-表二氧麦角甾-6,22-二烯-3β-醇（8）,啤酒甾醇（9）,β-谷甾醇亚油酸酯（10）.其中化合物1为新化合物,化合物5为新天然产物,其碳谱数据至今未曾报道.细胞毒活性表明化合物1-3对肿瘤细胞A549,B16F10,HL-60,MCF-7具有不同程度的抑制活性.其中新化合物1对乳腺癌细胞（MCF-7）的IC50值为14.9μmol·L-1、化合物3对肺癌细胞（A549）的IC50值为8.6μmol·L-1,两者活性强于阳性对照药顺铂.

Under the guidance of bioassay, five alkaloids, one chromone and four steroids were isolated from the Phomopsis longicolla HL-2232, a fungus isolated from a mangrove Bruguiera sexangula var. Rhynchopetala. Their structures were identified as 6-aminopurine-9-carboxylic acid methyl ester（1）, adenine riboside（2）, uridine（3）, N,N＇-diphenyl urea（4）,（2S,2＇R,3R,4E,8E,3＇E）-2-（2＇-hydroxy-3＇-octadecenoylamino）-9-methyl-4,8-octadecadiene-l,3-diol（5）, 2-（2＇S-hydroxypropyl）-5-methyl-7-hydroxychromone（6）, fortisterol（7）,（22E）-5α,8α-epidioxyergosta-6,22-dien-3β-ol（8）, cerevisterol（9） andβ-sitosteryl linoleate（10）. Among them, compound 1 was a new compound. Compound 5 was a new natural product, and its13 C NMR spectroscopic data has not reported until now. Compounds 1-3 showed inhibitory activities against B16F10, A549, HL-60 and MCF-7. Compounds 1 and 3 exhibited the higher inhibitory activities against MCF-7 and A549 than positive control cisplatin with IC50 values of 14.9 and 8.6 μmol·L-1, respectively.