糖槭多糖对RAW264.7炎症因子分泌的影响

Study on Anti- inflammatory Mechanism of Acer Saccharum Marsh Polysaccharide

目的:通过考察糖槭叶多糖(ASP-A)和糖槭果多糖(ASP-B)对脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7分泌细胞因子的影响,明确其抗炎作用机制。方法:MTT法测定不同浓度两类多糖对RAW264.7细胞的毒性作用,酶联免疫吸附试验(ELISA)检测细胞上清液中一氧化氮(NO)、肿瘤坏死因子-1α(TNF-1α)、前列腺素E2(PGE2)、白细胞介素-1β(IL-1β)的含量。结果:糖槭多糖能够抑制LPS诱导的RAW264.7细胞释放细胞炎症因子NO,TNF-1α,PGE2,IL-1β的分泌量。结论:ASP-A和ASP-B在浓度小于200 mg·L-1对RAW264.7细胞无显著毒性,且可以通过抑制细胞因子NO,TNF-1α,PGE-2,IL-1β的分泌发挥抗炎作用。

Objective： To investigate the effect of Acer saccharum Marsh polysaccharide（ ASP） on lipopolysaccharide（ LPS）-induced production of inflammatory cytokine in RAW264. 7 cell. Methods： The cytotoxicity of different ASP- A and ASP- B on RAW264. 7 cells was detected by MTT method. Levels of nitric oxide（ NO）,tumor necrosis factor- α（ TNF- α）,prostaglandin E2（ PGE2） and interleukin（ IL）- 1β in supernatant were assayed by ELISA. Results： ASP- A and ASP- B inhibited the secretions of NO,TNF- α,PGE2 and IL- 1β in LPS- induced RAW264. 7 cells. Conclusion： ASP- A and ASP- B in 200 mg·L- 1concentration has non- cytotoxic macrophages. The anti- inflammatory effects of ASP- A and ASP- B may be mediated in part by inhibiting the production of inflammatory mediators.