端粒酶表达与幽门螺杆菌感染关系的临床观察

Clinical Observation of Relationship between the Telomerase Expression and Helic Obacter Pylori Infection

目的 探讨端粒酶表达与幽门螺杆菌 (HP)感染在胃癌发生发展过程中的关系及临床意义。方法 用PCR- EL ISA法、病理组织学方法及快速尿素酶试验检测 111例各种胃病变的胃粘膜活检标本中端粒酶活性和 HP感染情况。结果 胃癌组织中端粒酶表达阳性率为 85 % ,明显高于癌前病变 (2 1.2 % ) ,癌周正常组织 (10 % )及浅表性胃炎(0 ) ,差异非常显著 (P<0 .0 1)。 HP感染率在胃癌组为 5 5 % ,癌前病变组为 6 3.5 %。胃癌及癌前病变端粒酶阳性率在 HP阳性组 (90 .9%、2 4.2 % )高于 HP阴性组 (77.8%、15 .8% ) ,但统计学处理无显著性差异 (P>0 .0 5 )。结论 本研究结果显示 ,检测端粒酶活性有助于胃癌的早期诊断。胃癌及癌前病变组织端粒酶表达与幽门螺杆菌感染无关。

Objective To observe the clinical significance and relationship between the telomerase expression and helicobacter pylori(H.pylori)infection in the genesis and development of gastric cancer.Methods To test the telomeras activi ty and H.pylori infection in 111 endoscopic specimens of gastric mucosa with PCR ELISA,histology and rapid urease test.Result The rate of telomerase positive in gastric cancer was 85%,significantly higher than that in precancerosis (21.2%),adjacent normal tissue (10%) and chronic superficial gastritis (0),(P<0.01).The rate of H.pylori infection in gastric cancer and precancerosis w as 55.5% and 63.5%,respectively.The rates of telomerase positive in gastric cancer and precancerosis in H.pylori positive groups (90.9%,24.2%) were higer than those in H.pylori negative groups,but there was no significant difference between them(P>0.05).Conclusions To test the telomerase activity was helpful to the diagnosis of the early gastric cancer.There was no relationship between the telomerase expression and H.pylori in gastric cancer.