承德产黄芩植株不同部位野黄芩苷含量分析

ANALYSIS OF SCUTELLARIN CONTENT IN DIFFERENT PARTS OF CHENGDE SCUTELLARIA BAICALENSIS GEORGI

目的:测定河北承德产黄芩植株不同部位野黄芩苷的含量。方法:采用高效液相色谱法测定河北承德隆化县黄芩GAP种植基地产黄芩植株不同部位(根、茎、叶、籽种壳)野黄芩苷的含量。色谱条件:柱温20.0℃、流动相甲醇:水:甲酸(20:80:0.4)、流速0.8ml/min、检测波长335nm。结果:承德产黄芩植株根、茎、叶、籽种壳中野黄芩苷的含量分别为0.94%、12.57%、10.54%、1.89%。结论:野黄芩苷在黄芩植株不同部位的含量分布为茎>叶>籽种壳>根。

Objective： To determine the scutellarin content in different parts of Chengde Scutellaria baicalensis Georgi. Methods： HPLC was used to determine the scutellarin content in different parts（root, stem, leaf, seedshell） of Scutellaria baicalensis Georgi produced in Chengde Longhua GAP planting base. The chromatographic condition： column temperature was 20.0℃, mobile phase was methanol-water-formic acid（20：80：0.4）, fl ow rate was 0.8ml/min, detection wavelength was 335 nm. Results： The scutellarin content in root, stem, leaf and seedshell of Scutellaria baicalensis Georgi produced in Chengde was respectively 0.94%, 12.57%, 10.54% and 1.89%. Conclusions： The scutellarin content distribution in different parts of Chengde Scutellaria baicalensis Georgi is stem〉leaf〉seedshell〉root.