红豆越橘提取物对慢性应激小鼠认知功能损伤的影响

Effects of lingonberry extraction on the mice cognitive function damaged by chronic stress

目的探究红豆越橘提取物对慢性应激损伤小鼠认知功能的影响。方法将雄性昆明小鼠随机分为空白对照组,应激模型组,氟西汀(4.4 mg·kg-1·d-1)阳性对照组及红豆越橘提取物低、中、高(50,100和200 mg·kg-1·d-1)剂量组,给药组采取连续慢性应激,并灌胃给药18 d,通过水迷宫实验测定小鼠认知记忆能力,测定与抗氧化功能相关的小鼠血清和脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,测定与应激损伤相关的小鼠血清和脑组织中神经递质去甲肾上腺素(NE)、5-羟基色胺(5-HT),糖皮质激素(GC)和乙酰胆碱酯酶(ACh E)活性。结果红豆越橘提取物能够改善应激模型小鼠的认知记忆能力;增加脑组织中SOD、GSH-Px酶活性,降低应激损伤产物MDA的含量;同时抑制血清中GC的升高及Ach E的激活,促进血清及脑组织中5-HT、NE含量的升高。结论红豆越橘提取物能够改善应激损伤小鼠的学习认知能力,其可能机制为通过增强抗氧化能力来降低氧化损伤并改善应激造成的神经递质水平紊乱。

Objective To study the effects of lingonberry extraction on mice cognitive impairment caused by chronic stress. Methods Kunming mice were randomly divided into six groups,which were control group,stress model group,the fluoxetine group（ dose of 4. 4 mg·kg^-1·d^-1）,lingonberry extraction low,medium and high dose group（ respectively 50,100 and 200 mg·kg^-1·d^-1 1）. All groups were given chronic uncertainty stress but the control group,and were intragastric administration for 18 days. Then the cognition of the mice was tested by using water maze,the contents of the SOD,GSH-Px,MDA and the activity of the neurotransmitters such as noradreline（ NE）,serotonin（ 5-HT）,glucocorticoids（ GC）,acetylcholinesterase（ Ach E） were measured by using kit.Results Lingonberry extraction improved the cognition and memory of the mice induced by chronic uncertainty stress,increased the content of the SOD and GSH-Px in mice brain, and decreased the content of oxidative damage markers MDA. Lingonberry extraction could also inhibit the increase of GC,inhibit the activity of Ach E in blood serum,elevated the content of 5-HT and NE in mice blood serum and brain. Conclusion Lingonberry extraction improved the cognition and memory of the mice induced by chronic uncertainty stress. The possible mechanism was that lingonberry enhanced the antioxidative ability of tissue and improved the disorder of neurotransmitter levels caused by chronic stress.