黄枝润楠组培快繁技术

The rapid propagation technique of Machilus versicolora in vitro

为了解决外植体易污染、易褐化、诱导分化难等问题,对黄枝润楠组培技术进行了探讨。试验结果表明:用75%酒精处理黄枝润楠的茎段15 s后,再用0.1%的升汞消毒10 min,能大大降低外植体污染率,外植体的存活率达60.0%。黄枝润楠的最佳诱导培养基为MS(改良)+6-BA 0.5 m/L+NAA 0.1 mg/L+蔗糖30 g/L+抗坏血酸1.0 g/L。

In order to avoid the explant browning, easily contaminated and difficultly differentiating and so on, we studied on the tissue culture technique of Machilus versicolora. The experiment results showed that after the stems being treated for 15 s with 75% alcohol ,and then by 0.1% HgCl2 disinfected 10 min,which greatly reduced the rate of explants pollution and the explant survival rate was up to 60.0%, The optimum inducing culture medium of M. versicolora was MS （modified） ＋ 6-BA 0.5 m/L ＋ NAA 0.1 mg/L ＋ sucrose 30 g/L ＋ ascorbic acid 1.0 g,/L.