流动注射化学发光法测定奈替米星

Flow injection chemiluminescence determination of netilmicin

目的建立流动注射化学发光法测定血浆中奈替米星的方法。方法在碱性介质中,奈替米星对铁氰化钾与鲁米诺化学发光体系具有明显的增敏作用,据此建立了一种测定奈替米星的流动注射化学发光新方法,对分析条件进行优化。通过方法学评价及对血浆奈替米星的测定,观察建立方法的性能。结果优化后的最佳检测条件是:鲁米诺浓度为10μmol/L,铁氰化钾的浓度为30μmol/L,Na OH的浓度为0.10mol/L,采样时间为5s,泵速为30r/min,负高压为630V。在确定的最佳实验条件下,化学发光增加强度与奈替米星的浓度在1.0×10^(-6)~5.0×10^(-5)g/m L范围内呈良好的线性关系,检测限为9.2×10^(-7)g/m L。对含3.6×10^(-6)g/m L奈替米星的溶液平行测定11次,测定结果的相对标准偏差为2.3%。结论本方法分析速度快、灵敏度高、分析成本低,可用于血浆中奈替米星的测定。

Objective To establish a new flow injection chemiluminescence method for the determination of netilmicin in plasma. Methods Based on that netilmicin could enhance the chemiluminescence intensity of luminol-potassium ferricyanide reaction system in alkaline condition, a new flow injection chemiluminescence method for the determination of netilmicin was developed, and the conditions of the analysis system were optimized. The performances of established method were determined by methodological evaluation and plasma netilmicin concentration assay. Results The optimized conditions of established method were 10μmol/L luminol, 30μmol/L potassium ferricyanide, 0.10mol/L NaOH, 5s sampling time, 30 r/min pump velocity and 630V negative voltage. Under the optimum conditions, the linear range for netilmicin was 1.0× 10-6-5.0× 10^-5g/mL （r=0.9912）, and the lower limit of detection was 9.2- 107g/mL. The relative standard deviation was 2.3% for the determination of 3.6× 10^-6g/mL netilmicin （n=11）. Conclusion The established method has the high sensitivity, the quick speed of analysis and low cost. It could be applied to determine the concentration of netilmicin in plasma samples.