诱导重编程过程中的表观遗传重塑

Remodeling of Epigenetic Landscape During Induced Somatic Cell Reprogramming

多能干细胞(pluripotent stem cell,PSC)是一类具有自我更新能力和多向分化潜能的细胞,具有广泛的临床应用前景.诱导性多功能干细胞(induced pluripotent stem cell,i PS cell)的获得,解决了传统方式中的细胞来源和伦理学等问题,从理论研究和应用上实现了体细胞重编程的重大突破,也为疾病发生机制研究、药物筛选、个性化药物选择、细胞治疗和再生医学等研究创造了难得的机会,从而开启了多能干细胞应用的新纪元.i PS过程中有很多问题尚未得到解决,尤其是诱导重编程的分子机制方面,这也是近年来干细胞领域研究的热点.其中如何实现表观遗传的重编程被认为是亟待解决的核心问题之一.本文结合我们的研究,主要介绍诱导重编程领域表观遗传修饰重塑机制的研究进展,并展望未来研究中大规模信息整合分析的重要性.

Pluripotent stem cells are capable of differentiation into different cell types, and therefore have great potential in clinical applications. The achievement of transcription-factor-induced pluripotent stem cells （ iPS cell） avoids ethical issues and offers an invaluable source of patient-specific pluripotent stem cells for disease modeling, drug screening, toxicology tests, personalized medicine selection, cell therapy and regenerative medicine. As a milestone, iPS technology initiates a new era of pluripotent stem cells. However, even untill now many problems especially the key molecular mechanism of epigenetic reprogramming during this process, are little understood. Uncovering the molecular mechanism of this unique platform would shed light on improving the reprogramming efficiency and iPSC quality, ultimately advancing their therapeutic applications. In this paper we will briefly introduce the significant progress in this field and technique and systematic biology in future research. attach importance to combining high-throughput sequencing