冷冻电镜技术在表观遗传学研究中的应用

Application of Cryo-Electron Microscopy on Epigenetic Studies

染色质装配、修饰和重塑复合体,以及它们和核小体、染色质等一起形成的超大分子复合体的精细结构解析,对于在原子水平揭示表观遗传信息建立、维持和调控的分子机制至关重要.近年来,迅速发展的冷冻电镜三维重构技术对于解析这些多亚基、大分子质量、柔性超大分子复合体的结构带来了很好的机遇.本文综述了冷冻电镜三维重构技术在表观遗传学相关的结构研究领域中的一些应用和进展.

The architecture and structural details of chromatin assembler, chromatin modifier, and chromatin remodeler, and their complexes with nucleosome and chromatin, are critical for revealing the molecular mechanisms for the establishment, maintenance and regulation of epigenetic information. The fast-developing cryo-electron microscopy （cryo-EM） provides an indispensible tool for the structure determiantion of the huge, multi-subunits, and flexible epigenetic regulation related macromolecular assemblies. In this review, we summarized the recent progresses on EM structural studies of macromolecules and complexes relating to epigenetic regulation.